一种基于SDS溶液高效提取土壤残留Bt蛋白的方法  

A Novel SDS-Buffer-Based Method for Extracting Bt Protein Residues From Soil

作　　者：方志翔[1] 孟军[1] 贺昭和[1] 刘标[1] 

机构地区：[1]环境保护部南京环境科学研究所国家生物安全重点实验室,江苏南京210042

出　　处：《生态与农村环境学报》2011年第1期84-87,共4页Journal of Ecology and Rural Environment

基　　金：转基因生物新品种培育科技重大专项(2008ZX08012-004;2008ZX08012-005;2008ZX08011-002)

摘　　要：通过对SDS浓度、孵育温度和孵育时间3种提取条件的研究,建立了一种基于SDS溶液的提取土壤中残留Bt蛋白的方法(简称SDS法)。在ρ(SDS)为2 g.L-1、孵育温度为50℃、孵育时间≥4 h条件下,采用SDS法可有效地从不同类型土壤样品中提取残留Bt蛋白,提取效果明显好于碳酸盐法、人造蠕虫肠道蛋白提取液法和PBST(phosphate buffer solution with Tween-20)法。A novel SDS based method for extracting Bt protein residues from the soil was developed based on studies on extraction conditions including concentration of SDS in the solution and temperature and duration of incubation.Results show that when SDS was 2 g·L^-1 in the solution at 50 ℃ for more than 4 hours it might extract effectively Bt protein residues from various types of soils,demonstrating better effect than the so-far known extraction methods,such as carbonate method,invertebrate gut fluid chemistry and PBST（phosphate buffer solution with Tween-20）.

关 键 词：SDS溶液 BT蛋白 提取 土壤 碳酸盐法 人造蠕虫肠道蛋白提取液法 PBST法 

分 类 号：X53[环境科学与工程—环境工程] Q-3[生物学]