孟鲁司特钠对哮喘大鼠肺组织、脾脏、胸腺NF-κB mRNA表达的影响及病理变化的研究  

The research of montelukast sodium to Asthma rats lung,spleen,thymus NF-κB mRNA expression and pathological changes

作　　者：陈伟[1] 潘家华[1] 倪陈[1] 楼皖玲[2] 

机构地区：[1]安徽医科大学附属省立医院儿科,安徽合肥230001 [2]安徽医科大学基础医学院,安徽合肥230022

出　　处：《临床肺科杂志》2011年第3期369-371,共3页Journal of Clinical Pulmonary Medicine

摘　　要：目的观察白三烯受体拮抗剂孟鲁司特钠对哮喘大鼠肺组织、脾脏、胸腺NF-κB mRNA的表达及病理变化的影响。方法建立哮喘大鼠模型后,将动物随机分成三组,正常对照组、哮喘组、孟鲁司特钠组。干预后处死动物,用原位杂交法检测肺组织、脾脏、胸腺淋巴细胞中NF-κB mRNA的表达及光镜观察肺组织、脾脏的病理变化。结果与正常对照组比较,哮喘组大鼠肺组织气道周围炎症明显,肺泡隔增宽。脾脏白髓淋巴细胞区中度增生。孟鲁司特组肺组织气道周围炎症减轻,肺泡隔增宽有所减轻,脾脏白髓淋巴细胞区轻-中度增生。哮喘组大鼠肺组织、脾脏、胸腺淋巴细胞中NF-κB mRNA的表达增加(P<0.05)。孟鲁司特钠组大鼠肺组织、脾脏、胸腺淋巴细胞中NF-κB mRNA的表达受抑(P<0.05)。结论孟鲁司特钠在支气管哮喘的发病机制中有一定的抗炎作用。Objective Observation of leukotriene receptor antagonist montelukast sodium in asthmatic lung tissue,spleen,thymus NF-κB mRNA expression and pathological change.Methods After the establishment of rat model of asthma,the rats were randomly divided into three groups,normal control group,asthma group,montelukast sodium group.After the intervention using in situ hybridization and method to detect lung,spleen,thymus lymphocytes of NF-κB mRNA expression and observed lung tissue,the pathological changes of the spleen by light microscopy.Results Compared with the control group,around asthma group mouse lung organization gas channel the inflammation is obvious,the pulmonary alveolus separates increases the width.Spleen white marrow lymphocyte area moderate proliferation.Montelukast around the group lung organization gas channel the inflammation reduces,the pulmonary alveolus separates increases the width to have reduces.Spleen white marrow lymphocyte area light-moderate proliferation.Asthmatic lung tissue,spleen,thymus lymphocytes the expression of NF-κB mRNA increased（P0.05）.Montelukast sodium lung tissue,spleen,thymus lymphocytes NF-κB mRNA expression was inhibited（P0.05）.Conclusion Montelukast sodium in the pathogenesis of bronchial asthma have a certain anti-in flammatory effect.

关 键 词：孟鲁司特钠 哮喘 NF-ΚB 

分 类 号：R562.25[医药卫生—呼吸系统]