复元活血汤含药血清对大鼠成骨细胞功能的影响  

Study on the effect of serum containing FuYuanHuoXue Recipe on the function of rat's osteoblasts in vitro

作　　者：于波[1] 崔宪春[2] 谢进[1] 

机构地区：[1]山东中医药大学附属医院,山东济南250011 [2]山东中医药大学,山东济南250014

出　　处：《中医正骨》2011年第1期17-21,共5页The Journal of Traditional Chinese Orthopedics and Traumatology

基　　金：山东省自然科学基金项目(Q2008C18)

摘　　要：目的:探讨复元活血汤含药血清对大鼠成骨细胞功能的影响及其作用机制。方法:取2月龄SD雄性大鼠12只,随机分为2组,每组6只,分别给予20 g.kg-1复元活血汤生药灌胃(含药血清组)和同等量的生理盐水灌胃(空白血清组),每天2次,14 d后腹主动脉采血。取新生SD大鼠2只,采用二次酶消化法消化幼鼠颅盖骨获取成骨细胞,采用血清药理学方法,分别采用含胎牛血清的培养液(胎牛血清组)、含复元活血汤生药灌胃大鼠血清的培养液(含药血清组)和含生理盐水灌胃大鼠血清的培养液(空白血清组)培养成骨细胞。分期测定体外培养的大鼠成骨细胞的增殖功能、分化功能、矿化功能及碱性磷酸酶含量。结果:①成骨细胞增殖功能。时间效应F=381.260,P=0.000;组别效应F=1 071.170,P=0.000;时间与组别交互效应F=22.500,P=0.000。②成骨细胞分化功能。时间效应F=159.950,P=0.000;组别效应F=62.120,P=0.000;时间与组别交互效应F=2.400,P=0.029。③成骨细胞矿化功能。时间效应F=115.500,P=0.000;组别效应F=4.370,P=0.082;时间与组别交互效应F=56.300,P=0.000。④成骨细胞碱性磷酸酶含量。时间效应F=2 175.670,P=0.000;组别效应F=1 201.370,P=0.000;时间与组别交互效应F=428.870,P=0.000。结论:复元活血汤能明显地促成骨细胞的增殖和分化功能,其作用可能与成骨细胞合成和分泌碱性磷酸酶有关。Objective：To study the effect of serum containing FuYuanHuoXue Recipe on the function of rat＇s osteoblasts in vitro and the mechanism of action.Methods：12 SD maleness rats aged 2 months were divided into two groups with 6 rats in each group at random,rats in one group（experimental group） undergone intragastric administration of FuYuanHuoXue decoction（20 g·kg-1） while equivalent normal saline was administrated in another group（control group） twice everyday,blood was obtained from abdominal aorta 14 days later.Osteoblasts were obtained from 2 neonatal rats and cultured respectively with fluid containing fetal bovine serum,serum from the rats in experimental group and control group.Function of osteoblasts was evaluated according to the measurement of proliferation,differentiation,mineralization and quantity of alkaline phosphatase（AKP）.Results：①Proliferation of osteoblast：time effect：F=381.260,P=0.000;effect between groups：F=1 071.170,P=0.000;reciprocal effect between time and group：F=22.500,P=0.000.②Differentiation of osteoblast：time effect：F=159.950,P=0.000;effect between groups：F=62.120,P=0.000;reciprocal effect between time and group：F=2.400,P=0.029.③Mineralization of osteoblast：time effect：F=115.500,P=0.000;effect between groups：F=4.370,P=0.082;reciprocal effect between time and group：F=56.300,P=0.000.④content of AKP：time effect：F=2 175.670,P=0.000;effect between groups：F=1 201.370,P=0.000;reciprocal effect between time and group：F=428.870,P=0.000.Conclusion：FuYuanHuoXue decoction can incite proliferation and differentiation of rat＇s osteoblast in vitro owing to the promotion in secretion of alkaline phosphatase.

关 键 词：复元活血汤 成骨细胞 碱性磷酸酶 

分 类 号：R285.5[医药卫生—中药学]