独一味化学成分及其活性的研究  

Constituents and bioactivities of Lamiophlomis rotata

作　　者：郝延军[1] 李琳[2] 陈沉[2] 桑育黎[2] 

机构地区：[1]辽宁省食品药品检验所,辽宁沈阳110023 [2]辽宁大学药学院,辽宁沈阳110036

出　　处：《中国中药杂志》2011年第4期465-467,共3页China Journal of Chinese Materia Medica

基　　金：辽宁省教育厅项目(2009A313)

摘　　要：目的:研究独一味的化学成分及其活性。方法:采用聚酰胺、硅胶、Sephadex LH-20柱色谱分离纯化化合物,运用理化鉴别及波谱分析技术鉴定化合物结构。采用醋酸扭体法、小鼠断尾止血时间和二甲苯致耳肿胀抗炎试验,测定化合物活性。结果:从独一味乙醇渗漉提取物中分得5个化合物,其中化合物8-epideoxyloganic acid为从该属植物中首次分得。与中成药独一味片组比较,8-epideoxyloganic acid的低、高剂量组均有明显的镇痛、止血作用,高剂量组有明显的抗炎作用。结论:8-epideoxyloganic acid为独一味中镇痛、止血、抗炎的有效成分之一。Objective： To investigate the chemical constituents from Lamiophlomis rotata and the bioactivities of 8-epideoxyloganic acid. Method： The constituents were isolated by using a combination of various chromatographic techniques including column chromatography over ployamide, silica gel and Sephadex LH-20. Structures of the isolates were identified by spectroscopic data analysis. Bioactivities were screened by using models in vivo. Result： Five constituents were isolated. 8-epideoxyloganic acid was isolated for the first time in L. rotata and also in lamioplomis genus. 8-epideoxyloganie acid could significantly inhibit aectic acid-induced twisting times and slower the time of homeostatsis, also inhibit xylene-induced ear edema in mice. Conclusion： 8-epideoxyloganic acid possesses bioactivities of analgesia , homeostasis and anti-inflammatory.

关 键 词：独一味 8-epideoxyloganic ACID 镇痛 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]