HPLC-ELSD法同时测定知母药材中2种皂苷含量  

Simultaneous determination of two saponnins in Anemarrhenae Rhizoma by HPLC-ELSD

作　　者：陈千良[1,2] 孙小明[3] 王文全[4] 马长华[4] 

机构地区：[1]西北大学生命科学学院西部资源生物与现代生物技术教育部重点实验室,陕西西安710069 [2]西北大学陕西省生物医药重点实验室,陕西西安710069 [3]中国科学院兰州化学物理研究所西北特色植物资源化学重点实验室,甘肃兰州730000 [4]北京中医药大学中药学院,北京100102

出　　处：《中国中药杂志》2011年第4期474-477,共4页China Journal of Chinese Materia Medica

基　　金：国家"十五"重大科技专项(2001BBA701A62-13);西北大学科研启动项目(OKYQDF159);国家药典委员会2010年版<中国药典>一部标准研究项目(YZ-196~198)

摘　　要：目的:用HPLC-ELSD同时测定知母药材中2种皂苷的含量。方法:采用Kromasil C18柱(4.6 mm×250 mm,5μm),以甲醇-水梯度洗脱,流速1 mL.min-1,柱温为30℃。蒸发光散射检测器漂移管温度50℃,蒸发温度70℃,以氮气为雾化气,压力为1.03×105 Pa。结果:知母皂苷C在0.310～3.10μg,知母皂苷AⅢ在0.323～3.23μg,进样质量的对数值与峰面积的对数值呈良好的线性关系。知母皂苷C回归方程为lgA=1.254 2lgM+5.734 7,r=0.999 5,测定的平均回收率(n=6)98.1%,RSD2.0%;知母皂苷AⅢ回归方程为lgA=1.328 4lgM+5.937,r=0.999 6,测定的平均回收率(n=6)97.3%,RSD1.5%。结论:建立的含量测定方法准确、快速,是控制知母药材质量较理想的方法。对我国北方主要知母产地药材中两种皂苷含量测定和比较表明不同产地野生知母质量差异较大。Objective： To establish an HPLC-ELSD method for determination of Anemarsaponin C and Anemarsaponin Am in Anemarrhenae Rhizoma. Method： Kromasil CI8 column（4.6 mm ×250 mm, 5 μm） was used as stationary phase. Mobile phase was methanol-water gradient with the flow rate of 1 mL min-1 ; the temperature of the drift tube and evaporation was 50 ℃ and 70 ℃ respectively. The gas pressure was 1.03 × 105 Pa. Result： There are good linearity in the range 0. 310-3.10 μg of anemarsaponin C （ lgA = 1. 254 21gM ＋ 5. 734 7, r = O. 999 5 ） and in the range O. 323-3.23 μg （ lgA = 1. 328 41gM ＋ 5. 937, r = 0. 999 6） of anemarsaponin AⅢ. The average recovery of anemarsaponin C and anemarsaponin Am was 98. 1% with RSD 2.1% and 97.3% with RSD 1.5% （ n = 6） respectively. Conclusion： The method is rapid and accurate. It is suitable for quality control of Anemarrhenae Rhizoma. The resuh of determination reveals that the quality of Anemarrhenae Rhizoma from different places of north China are of notable difference.

关 键 词：知母 高效液相蒸发光检测 知母皂苷C 知母皂苷AⅢ 含量测定 

分 类 号：R284.1[医药卫生—中药学]