气-质联用结合衍生化法分析血浆中甲基苯丙胺和氯胺酮  

Simultaneous determination of methamphetamine and ketamine in plasma by GC/MS with derivation

作　　者：王皓玉[1] 王玉瑾[1] 贾娟[1] 曹洁[1] 尉志文[1] 贠克明[1] 

机构地区：[1]山西医科大学法医学院,山西太原030001

出　　处：《中国医院药学杂志》2011年第4期264-267,共4页Chinese Journal of Hospital Pharmacy

基　　金：山西省自然科学基金资助项目(编号:20011066;2007011105);"十一五"国家科技支撑计划项目(编号:2007BAK26B05);山西医科大学博士启动基金资助项目(编号:03200814)

摘　　要：目的：考察家兔血浆中甲基苯丙胺和氯胺酮同时衍生化后GC／MS检测分析结果。方法：血浆样品中加入内标物丙基解痘素（SKF525A）后碱化，乙醚萃取，三氟醋酸酐（TFA）衍生化，GC／MS全扫描定性、内标法和工作曲线法定量分析。结果：甲基苯丙胺和氯胺酮在血浆中的线性检测范围分别为0．0100～10．0μg·mL^-1和0.0100-20．0μg·mL^-1；方法回收率为95．26％～101．10％；日内及日间相对标准差均小于15％。结论：建立了衍生化后同时测定血浆中甲基苯丙胺及氯胺酮的GC／MS定性定量分析方法，该法简便、灵敏、重复性好，适用于甲基苯丙胺和氟胺酮混合滥用中毒案例的快速鉴定。OBJECTIVE To establish a method for simultaneous determination of methamphetamine and ketamine in plasma derivatized with TFA by GC＂MS. METHODS After adding internal standard （IS） SKF525A, plasma samples were alkalized, extracted with ether and derivatized with trifluoroacetic anhydride （TFA）. Optimized qualitative analysis employed full-scan mode and quantitative analysis by internal standard method and calibration curves by GC/MS. RESULTS The linear range for methamphetamine and ketamine in plasma were 0. 010 0 - 10. 0μg·mL^-1 and 0. 010 0 - 20. 0 μg·mL^-1 respectively. The extraction recovery rates of the target compounds in plasma were more than 66% and were stable relatively. The analytfcal recovery rates were 95. 26% - 101.10% and inte〉day and intra day relative standard deviation of precision were less than 15%. CONCLUSION The CR2/MS method established for the simultaneous determination of methamphetamine and ketamine in biological samples derivatized with TFA was proved to be simple, sensitive and reproducible and could be applied to analyze intoxi- cation cases of methamphetamine and ketamine mixed abusing.

关 键 词：甲基苯丙胺 氯胺酮 衍生 气-质联用法 

分 类 号：R969.3[医药卫生—药理学]