分光光度法测定大黄中总蒽醌  

Determination of Total Anthraquinones in Rhubarb by Spectrophotometry

作　　者：鲁彦[1] 郭建宇[2] 朱贤[1] 吴毓倩[1] 

机构地区：[1]上海应用技术学院化学与环境工程学院,上海200235 [2]上海师范大学生环学院,上海200234

出　　处：《理化检验（化学分册）》2011年第1期21-22,26,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：上海市教委科技基金项目(06OZ008;06DZ009);上海市教育发展基金会晨光计划项目(2007CG69);国家自然科学基金项目(No.20905049)

摘　　要：大黄试样经乙醇-水（6＋4）溶液回流提取3次,每次0.5 h。以母核1,8-二羟基蒽醌为标准样品,在428 nm测定波长下,采用分光光度法测定大黄中总蒽醌的含量。在优化的试验条件下,1,8-二羟基蒽醌的质量浓度在5.0-25.0 mg·L^-1范围内与其吸光度呈线性关系。用此方法测定大黄中总蒽醌含量,加标回收率在98.7%-99.8%之间。Sample of rhubarb was extracted with mixture of ethanol and water（6＋4） by refluxing for 3 times with 0.5 h for each time.The content of total anthraquinones in the extract was determined by spectrophotometry using 1,8-dihydroxy-anthraquinone as standard sample at the wavelength of 428 nm.Under the optimum condition,linear relationship between values of absorbance and mass concentration of 1,8-dihydroxy-anthraquinone was kept in the range of 5.0-25.0 mg·L^-1.Test for recovery was made by standard addition method,and values of recovery obtained were in the range of 98.7%-99.8%.

关 键 词：分光光度法 回流提取 大黄 总蒽醌 

分 类 号：O657.32[理学—分析化学]