花生青枯病内生拮抗细菌的鉴定、抗菌活性及其田间防效  

Identification,Antimicrobial Activity and Field Control Efficacy of an Endophytic Bacteria Strain against Peanut Bacterial Wilt

作　　者：王小兵[1,2,3] 骆永明[1,2] 刘五星[1] 李振高[1] 

机构地区：[1]中国科学院土壤环境与污染修复重点实验室,南京土壤研究所,南京210008 [2]中国科学院研究生院,北京100049 [3]扬州大学环境科学与工程学院,扬州225009

出　　处：《中国生物防治学报》2011年第1期88-92,共5页Chinese Journal of Biological Control

基　　金：国家自然科学基金重点项目(40432005);中国科学院知识创新工程重要方向项目(CXTD-Z2005-4)

摘　　要：从病区健康花生植株茎秆内分离到1株对花生青枯病菌Ralstonia solanacearum有强拮抗作用的内生细菌,命名为BZ6-1菌株。拮抗试验表明,该菌具有较广抗菌谱,对多种植物病原真菌有较强的抑制作用。形态观察和16SrDNA同源性分析初步鉴定为解淀粉芽孢杆菌Bacillus amyloliquefaciens。该菌的最佳培养基组成为:糖蜜10g.L-1,蛋白胨0.5g.L-1,酵母膏0.5g.L-1。最适发酵条件为pH8.5、装液量50mL/250mL、温度25℃、转速200r.min-1、培养时间21h,在此条件下拮抗圈直径达34.0mm。田间试验结果表明BZ6-1菌株对花生青枯病防效达62.3%。An endophytic bacteria strain BZ6-1,isolated from the stem of healthy peanut plants in Ralstonia solanacearum-infested fields,suppressed R.solanacearum greatly and was of broad spectrum against plant pathogenic fungi.According to its morphology and 16S rDNA sequence,the strain BZ6-1 was preliminarily identified as Bacillus amyloliquefaciens.Diameter of inhibition zone reached 34.0 mm under the optimal fermentation conditions for antibiotic production,which was molasses 10 g,peptone 0.5 g and yeast extract 0.5 g in l L medium at pH 8.5,temperature 25 ℃,rotation rate 200 r·min-1,medium amount 50 mL in 250 mL flask and incubation period 21 h.Field trials demonstrated that the control efficiency of strain BZ6-1 against peanut bacterial wilt disease was 62.3%.

关 键 词：花生青枯病 解淀粉芽孢杆菌 内生细菌 抗菌活性 田间防效 

分 类 号：S435.652[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]