辣椒根腐病内生拮抗细菌筛选及菌株Hj33-7的鉴定和抗菌活性  

Screening of Pepper Endophytic Bacterial Antagonists against Fusarium oxysporum and Identification and Antagonistic Activities of Strain Hj33-7

作　　者：祁之秋[1] 李兴海[1] 王英姿[1] 谷祖敏[1] 纪明山[1] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161

出　　处：《中国生物防治学报》2011年第1期93-98,共6页Chinese Journal of Biological Control

基　　金："十一五"国家科技支撑计划项目(2006BAD07B02);校博士后经费

摘　　要：从采自黑龙江和辽宁辣椒根腐病区的健康辣椒植株体内分离到141株内生细菌。对峙培养发现10株内生细菌对辣椒根腐病菌Fusarium oxysporum有较强的抑制作用,各菌株抑菌带宽均在7mm以上。10株拮抗细菌对辣椒根腐病的防治效果从25%到75%,其中,菌株Hj33-7、Hj33-6、Hj33-4及Hgu23-2对辣椒根腐病的防治效果分别达到75%、75%、66.6%和66.6%。经形态、生理生化特征和分子生物学等测定分析,将菌株Hj33-7初步鉴定为地衣芽孢杆菌Bacillus licheniformis。Hj33-7发酵滤液对辣椒根腐病菌菌丝生长与形态、孢子萌发均有较强的抑制活性。141 strains of endophytic bacteria were isolated from healthy pepper plants in severely infested plots in Heihongjiang and Liaoning Provinces.Their antagonistic activities to Fusarium oxysporum were tested by dual-culture tests.The results showed that 10 strains from root,stem and fruit showed strong antagonistic activities to F.oxysporum with the width of inhibition zones bigger than 7mm.The efficacy of 10 strains of the endophytic bacteria to pepper root rot disease ranged from 25% to 75%,and the control efficiency of Hj33-7、Hj33-6、Hj33-4 and Hgu23-2 were 75%、75%、66.6% and 66.6%,respectively.Based on the morphological,physiological and biochemical characteristics and 16S rDNA sequences analysis,strain Hj33-7 was identified as Bacillus licheniformis.The fermentation filtrate of Hj33-7 showed strong antagonistic activities to the hyphal growth,hyphal morphology and spore germination of F.oxysporum.

关 键 词：根腐病菌 内生细菌 筛选 鉴定 抗菌活性 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S436.418[农业科学—植物保护]