水稻内生细菌B196的鉴定及其对水稻纹枯病的防治作用  

Identification of Rice Endophytic Bacterial Strain B196 and Its Control Effect on Rice Sheath Blight

作　　者：农倩[1] 陈雪凤[1] 黎起秦[1] 袁高庆[1] 林纬[1] 黄永禄[1] 

机构地区：[1]广西大学农学院,南宁530004

出　　处：《中国生物防治学报》2011年第1期99-103,共5页Chinese Journal of Biological Control

基　　金：广西自然科学基金(桂科自0728012);广西大学研究生教育创新项目(2008105930904M023)

摘　　要：从水稻体内分离得到的细菌菌株B196对水稻纹枯病菌Rhizoctonia solani的生长有较强抑制作用。通过形态学和生理生化鉴定及16S rDNA序列分析,将菌株B196鉴定为巨大芽孢杆菌Bacillus megaterium。采用浸种和喷雾的方法将菌株B196接种到水稻植株后,均能在稻株体内回收到该菌株,表明菌株B196能在水稻体内定殖。菌株B196对水稻纹枯病的盆栽和田间防效分别为64.29%和55.13%。A bacterium strain B196,isolated from the rice plants,could inhibit the growth of Rhizoctonia solani.According to its morphological,physiological and biochemical characteristics and 16S rDNA sequence analysis,the strain B196 was identified as Bacillus megaterium.This strain could colonize in rice plants after inoculating with seed dipping and spraying.Control effect of strain B196 on rice sheath blight was 64.29% in pot and 55.13% in fields.

关 键 词：内生细菌 鉴定 定殖 水稻纹枯病 防治效果 

分 类 号：S435.111[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]