保护剂和再水化剂对冷冻干燥保存的生防菌YT11的存活率及生防效果的影响  

Impact of Protective Agents and Rehydration Media on Viability and Efficacy of the Freeze-dried Biocontrol Agent Pantoea ananatis Strain YT11

作　　者：詹祎[1] 王云鹏[1] 郭坚华[1] 

机构地区：[1]南京农业大学植物保护学院植物病理学系/江苏省生物源农药工程中心/农业部作物病虫害监测与防控重点开放实验室,南京210095

出　　处：《中国生物防治学报》2011年第1期110-114,共5页Chinese Journal of Biological Control

基　　金：农业部行业(农业)科研专项(200903052);江苏省科技创新与成果转化专项引导资金项目(BY2009157)

摘　　要：本文研究了多种保护剂和再水化剂对生防菌成团泛菌Pantoea ananatis菌株YT11在冷冻干燥保存中存活率的影响,比较了不同保存温度对YT11贮藏稳定性和生防活力的差异。结果表明,5%蔗糖是最佳的保护剂,冷冻干燥后菌株YT11存活率达90%以上。以5%蔗糖作保护剂时,5%脱脂奶粉为最佳再水化剂,菌株YT11复苏率达100%。贮藏试验结果表明,4℃贮存的冷冻干燥粉剂的菌株存活率显著高于22℃下的存活率。对保存24周后的菌剂进行温室生防试验,结果显示,4℃和22℃下保存的菌剂对番茄青枯病的防效分别达到71.57%和50.98%。因此,以5%蔗糖为保护剂和5%脱脂奶粉为再水化剂,对成团泛菌菌株YT11进行冷冻干燥处理,再将冷冻干燥后的菌株于4℃贮存,可有效保证生防菌的生防活力。The effect of different protective agents and rehydration media on the viabilities of the biocontrol agent Pantoea ananatis strain YT11 subjected to freeze-drying,its subsequent survival rates during storage under different temperatures,and the biocontrol efficacies of the freeze-dried cells were investigated.Results indicated that 5% sucrose gave the greatest protection to YT11 with cell viability more than 90% during freeze-drying,and 5% non-fat skim milk as rehydration media gave the highest survival rate reaching 100%.Freeze-dried cells stored at 4℃ survived more than those at 22℃.YT11 powder formulation stored for 24 weeks at 4℃ and 22℃ gave control efficacies by 71.57% and 50.98%,respectively.

关 键 词：冷冻干燥保护剂 再水化剂 成团泛菌 存活率 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S482.7[农业科学—植物保护]