甲泼尼龙对急性百草枯中毒大鼠肺损伤病理变化的影响  

Effects of methylprednisolone on lung pathological changes in rats with paraquat intoxication

作　　者：赵文鹏[1] 马玉英[2] 豆周林 王占青[1] 

机构地区：[1]新疆石河子大学医学院,832002 [2]新疆石河子大学医学院第一附属医院急诊科,硕士生导师832008

出　　处：《新医学》2011年第1期29-31,F0003,共4页Journal of New Medicine

摘　　要：目的:通过研究甲泼尼龙对急性百草枯中毒大鼠肺病理学改变的作用,探讨甲泼尼龙对百草枯中毒大鼠早期肺损伤的保护作用。方法:60只Wistar大鼠,随机分为4组,正常对照组(A组)、模型组(B组)、甲泼尼龙15 mg/kg组(C组)、甲泼尼龙5 mg/kg组(D组)。B、C、D组采用百草枯22 mg/kg腹腔注射一次性染毒,C、D组从染毒后2 h开始,分别予腹腔注射甲泼尼龙15 mg/kg和5 mg/kg,每日1次,同期A、B组用等体积无菌生理盐水腹腔内注射,每日1次,至处死前。染毒后24、72、168 h分批处死大鼠,每组每时间点6只,摘取肺脏,制备肺组织标本,光镜下观察肺组织的病理学改变并行肺损伤评分。结果:光镜下C、D组大鼠肺组织的病理学改变比B组轻,其中以D组减轻最为明显。染毒后24 h C、D组的肺损伤评分明显低于B组(P均<0.05);72 h D组的肺损伤评分明显低于B组(P<0.05),而B、C组的肺损伤评分比较差异无统计学意义(P>0.05);168 h B、C、D组的肺损伤评分均明显高于A组(P<0.05),其中C、D组的肺损伤评分明显低于B组(P均<0.05),C、D组的肺损伤评分比较差异无统计学意义(P>0.05)。结论:甲泼尼龙对急性百草枯中毒大鼠的肺损伤具有保护作用,且小剂量(5 mg/kg)甲泼尼龙优于常规剂量(15 mg/kg)。Objective：To study the effects and mechanism of methylprednisolone on lung pathological changes in rats with paraquat intoxication.Methods：Sixty Wistar rats were randomly divided into 4 groups：control group（Group A）,intoxication group（Group B）,15 mg/kg Methylprednisolone group（Group C） and 5 mg/kg Methylprednisolone group（Group D）.Intoxication was induced by intraperitoneal injection of paraquat dissolvent（22 mg/kg） in group B,C,D.Intraperitoneal injection of methylprednisolone（5 mg/kg and 15 mg/kg） was given daily in Group C and D,respectively,while only saline was injected in the other two groups.Six rats in each group were sacrificed at 24th,72nd and 168th hour,for pathological observation of lung tissues by light microscope.Results：Significantly milder pathological change of acute lung injury was observed in Group C and D,compared with that in Group B（P〈0.05）.Significantly lower lung injury score in Group D was revealed at 24th and 72nd hour,compared with that in Group B,whereas only significantly lower score presented in Group C at 24th hour（P〈0.05）.The lowest lung injury score was found in Group A among all 4 groups（P0.05）.Furthermore,scores were significantly lower in Group C and Group D than that in Group B（P〈0.05）,though no significant difference was found between the two treatment groups.Conclusion：Methylprednisolone has protective effect on lung injury in rats with paraquat intoxication,with superior efficacy observed in low dosage than in higher dosage.

关 键 词：甲泼尼龙 百草枯 急性中毒 病理 肺损伤 

分 类 号：R595.4[医药卫生—内科学]