SIM对人骨形态发生蛋白-2诱导大鼠异位成骨的X射线学研究  

The radiology research of Simvastat on Ectopic Bone Formation of rat Induced by Recombinant Human Bone Morphogenetic

作　　者：王岩 陈国栋 庞天舒 

机构地区：[1]辽河油田中心医院放射线科,124000

出　　处：《中国实用医药》2011年第2期31-32,共2页China Practical Medicine

摘　　要：目的在重组人骨形态发生蛋白-2(rhBMP-2)诱导异位成骨过程中,局部应用不同浓度的辛伐他汀(SIM),探讨SIM对rhBMP-2诱导成骨的作用。方法①以牛松质骨为原料经过脱脂、脱蛋白等步骤制备牛骨基质明胶载体,将辛伐他汀和rhBMP-2分别与BMG载体复合;②将45只雄性SD大鼠随机分为3组。A组:高剂量实验组;B组:低剂量实验组;C组:对照组;③将复合体植入大鼠股后部肌袋中,术后15d、30d、60d对实验大鼠进行大体观察、X线检测。结果①大体观察:各组组织块均随着时间延长而变硬,范围变大;②X线结果,各组植入部位术后15d、30d未见显影,于术后60d各组可见不同密度的影像,B组和C组钙化明显,成高密度影像;A组成低密度影像。成骨区灰度值A组明显低于B组和C组。结论高浓度辛伐他汀可明显抑制rhBMP-2的促进成骨作用;低浓度辛伐他汀的抑制作用则不明显;证实了rhBMP-2在促进成骨方面有重要作用。Objective To investigate the synergistic effect of different concentration Simvastat（SIM）on the bone induction of recombinant human bone morphogenetic protein-2（rhBMP-2）through local application of SIM in the osteoinductive process of BMP.Methods ①Prepare the bone matrix galatin（BMG）carrier as the raw materiar from cattle spongy bone by the procedure of degrease, deproteinization and so on.To compound the BMG carrier with SIM、rhBMP-2 or both.②Forty-five male SD rat were divided into two experimental groups and control group at random,and BMG composite was implanted into the right thigh muscle pouch of each group. group A：the BMG carrier contain 15% weight ratio of SIM and 0.25 mgrhBMP-2 as high dose experimental group;group B：the BMG carrier contain 2.5% weight ratio of SIM and 0.25 mgrhBMP-2 as low dose experimental group; group C：the BMG carrier just contain 0.25 mgrhBMP-2 as control group（CG）. ③At dl5, d30 and d60 after implantation, new bone formation was measured by generally, radiographically of the three groups.Results ①The carriers of each group become hard and extensive. The effect of wet weight：the groups of A, B, C were different, which the group C has the strongest effection of all. ②X-ray results：Each group can be seen the developing after 60 days from operation, which group B and Group C have obviously calcification.The detection of gray scale on the zone of ossification：group B and Group C are much higher than group A.Conclusion The Simvastat of high concentration group can inhibit the osteoogenesis of rhBMP-2 obviously,however,the low concentration group have not obviously inhibitory action.Confirm the important effectiveness of rhBMP-2 on the osteoinductive process.

关 键 词：辛伐他汀 重组人骨形态发生蛋白-2 诱导 成骨 X射线放射学 

分 类 号：R965[医药卫生—药理学] R816.8[医药卫生—药学]