5株虫生真菌对淡色库蚊幼虫的致病力研究  

Study on the pathogenicity of five strains of entomopathogenic fungi to Culex pipiens pallens larvae

作　　者：刘丽娟[1] 韦玮[2] 张本光[1] 代玉华[1] 王海防[1] 程鹏[1] 王怀位[1] 公茂庆[1] 

机构地区：[1]山东省寄生虫病防治研究所,济宁272033 [2]济宁市第一人民医院

出　　处：《中国媒介生物学及控制杂志》2011年第1期11-13,共3页Chinese Journal of Vector Biology and Control

基　　金：山东省科技发展计划项目(2010GSF10207);山东省医学科学院科技资助项目(2009-08)~~

摘　　要：目的测定菌株对淡色库蚊幼虫的致病力,筛选出高致病力菌株,为淡色库蚊的防治提供理论依据。方法选择生物防治中常用的5株菌株,测定不同浓度的孢子悬浮液对淡色库蚊幼虫的累计死亡率,计算半数致死浓度(LC50)。结果菌株GIM3.428、GIM3.45、GIM3.431、GIM3.95以及GIM3.436对淡色库蚊幼虫均具有致病性,试验10d,淡色库蚊幼虫累计死亡率分别为100%、82%、79%、73%和57%,菌株对淡色库蚊幼虫的LC50分别为2.98×102、1.41×103、1.26×104、1.02×104、2.07×105孢子/ml。结论菌株GIM3.428对淡色库蚊幼虫的致病力最高,可将其应用于淡色库蚊的防治。Objective To test the pathogenicity of 5 strains of entomopathogenic fungi to Culex pipiens pallens larvae and select the highly pathogenic strain against Cx. pipiens pallens, providing a scientific basis for the prevention and control of the insect. Methods The five fungal strains that are commonly used in biological control were used for the detection of the cumulative motality rates of Cx. pipiens pallens larvae caused by different concentrations of fungal spore suspension, with the median lethal concentration （LC50） values worked out. Results Evaluated based on the growth rate and sporulation, all of the five strains showed different degrees of pathogenicity against Cx. pipiens pallens larvae. At 10 days, the five strains GIM3.428, GIM3.45,GIM3.431, GIM3.95 and GIM3.436 resulted in a cumulative mortality rate of 100%, 82%, 79%, 73% and 57%, with the LC50 being 2.98×102、1.41×103、1.26×104、1.02×104、2.07×105 conidia/ml, respectively. Conclusion The fungal strain GIM3.428, which is of the highest pathogenicity against Cx. pipiens pallens larvae, can be used for the control of the insect.

关 键 词：淡色库蚊 虫生真菌 菌株 致病力 

分 类 号：R384.1[医药卫生—医学寄生虫学] R379[医药卫生—基础医学]