阿维菌素原药对昆明小鼠免疫功能的影响  

Immunotoxicity of avermectin on Kunming mice

作　　者：左派欣[1] 徐颖[1] 陈淑芬[1] 呼亚伟[1] 郭金铭[1] 杨卫超[1] 

机构地区：[1]河北省疾病预防控制中心卫生毒理所,石家庄050021

出　　处：《中国媒介生物学及控制杂志》2011年第1期26-28,共3页Chinese Journal of Vector Biology and Control

摘　　要：目的探讨阿维菌素原药对昆明小鼠细胞免疫功能、体液免疫功能和非特异性免疫功能的影响。方法阿维菌素设3个剂量组20、10、5mg/kg和阴性对照组,连续给昆明小鼠染毒28d;T淋巴细胞增殖和迟发型变态反应(DTH)检测细胞免疫功能;抗体生成细胞数(PFC)和半数溶血值(HC50)测定检测体液免疫功能;腹腔巨噬细胞吞噬鸡红细胞、碳粒廓清试验和NK细胞活性测定检测非特异性免疫功能。结果 20mg/kg组小鼠T淋巴细胞增殖能力为0.76(阴性对照为0.86),HC50为128.45(阴性对照为140.96),PFC为89.21×106(阴性对照为113.33×106);20和10mg/kg组碳廓清吞噬指数分别为5.59、5.61(阴性对照为7.96),鸡红细胞吞噬指数分别为0.86、0.90(阴性对照为1.05),以上结果均比阴性对照组明显降低,且差异有统计学意义﹙P<0.01﹚。结论阿维菌素原药对昆明小鼠在细胞免疫功能、体液免疫功能和非特异性免疫功能方面均有抑制作用。Objective To measure the impact of avermectin on the cellular immunity, humoral immunity and nonspecific immunity of Kunming mice. Methods The subject mice were assigned to the experimental groups to receive avermectin at doses of 20, 10, 5 mg/kg for 28 days and a blank control group. The cellular immunity was evaluated in terms of T lymphocyte proliferation and delayed type hypersensitivity （DTH）, the humoral immunity estimated with plaque forming cell assay （PFC） and half hemolysis value method （HC50）, and the nonspecific immunity measured based on peritoneal macrophages phagocytosis for chicken red blood cells, carbon clearance test and NK cell activity. Results At a dose of 20 mg/kg, the T lymphocyte proliferation was 0.76 （in contrast to 0.86 in the negative control）, the half hemolysis value 128.45 （versus 140.96 in the negative control）, and the PFC 89.21×106 （versus 113.33×106）. In the 20 mg/kg and 10 mg/kg medication groups, the carbon clearance indices were 5.59 and 5.61 （versus 7.96）, and the peritoneal macrophage phagocytosis levels for chicken red blood cells were 0.86 and 0.90 （versus 1.05）. Reduction was observed in all of the above function indicators compared to the control group, with significant difference （P0.01）. Conclusion Avermectin inhibits the celluar immunity, humoral immunity and nonspecific immunity of Kunming mice.

关 键 词：阿维菌素 细胞免疫 体液免疫 非特异性免疫 

分 类 号：S481.9[农业科学—农药学]