布氏田鼠对溴敌隆的敏感性测定  

The detection of the susceptibility of Lasiopodomys brandti to bromadiolone

作　　者：曹煜[1] 牛海峰[2] 田治中[2] 张进华[3] 董天义[1] 

机构地区：[1]中国农业科学院植物保护研究所,北京100094 [2]河北农业大学现代科技学院 [3]中国科学院动物研究所

出　　处：《中国媒介生物学及控制杂志》2011年第1期59-61,共3页Chinese Journal of Vector Biology and Control

摘　　要：目的测定布氏田鼠对抗凝血灭鼠剂溴敌隆的敏感性,为检验该鼠的抗药性提供参数。方法实验室单鼠笼养无选择摄食试验:雌雄分开,随机分组,按5个不同食毒期供给浓度为0.0005%的溴敌隆毒饵;用Bliss计算机程序,计算不同食毒期与对应死亡率之间的关系。结果布氏田鼠对溴敌隆的敏感性性别之间差异无统计学意义(Pb>0.05,PLFP50>0.05);雌雄数据合并计算,得毒力回归线y=3.35+4.81x,LFP50和LFP99及其95%置信限分别为2.20(1.88～2.58)d和6.71(4.48～10.05)d。结论按世界卫生组织推荐的以致死99%敏感靶标鼠种的食毒期(LFP99)取整天数作为抗药性检验标准,布氏田鼠摄食浓度为0.0005%溴敌隆毒饵10d存活即为抗性鼠。Objective To determine the susceptibility of Lasiopodomys brandti to bromadiolone for providing the base data on its resistance to bromadiolone. Methods The males and females were separated and divided into groups at random. No-choice feeding tests were carried out with 0.0005% bromadiolone bait at five different feeding days. The relationship between feeding days and their corrresponding mortality rates was analyzed by Bliss software. Results There was no significant difference （Pb0.05, PLFP500.05） between male and female L. brandti in terms of their susceptibility to bromadiolone, with the LFP50 and LFP99 of bromadiolone to L. brandti being 2.20（1.88-2.58） days and 6.71（4.48-10.05） days respectively. Conclusion Based on the criterion recommended by WHO, LFP99 value of bromadiolone to susceptible rodents is used as the resistance diagnose criterion. L. brandti is considered to be resistant when it is still alive after being fed on the bait containing 0.0005% bromadiolone for 10 days.

关 键 词：布氏田鼠 抗凝血灭鼠剂 溴敌隆 敏感性 

分 类 号：S443[农业科学—农业昆虫与害虫防治] S481.4[农业科学—植物保护]