部分竹类植物遗传变异的AFLP,ISSR和SRAP分析  

Analysis of genetic variation of some bamboo species by AFLP,ISSR and SRAP

作　　者：娄永峰[1] 杨海芸[1] 张有珍 李晓芬[1] 林新春[1] 方伟[1] 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300 [2]临安市林业科学研究所,浙江临安311300

出　　处：《福建林学院学报》2011年第1期38-43,共6页Journal of Fujian College of Forestry

基　　金：浙江省重大科技专项基金资助项目(2006C12008);浙江省林业厅基金资助项目(08A09)

摘　　要：采用AFLP和ISSR分子标记技术对簕竹属4个竹种的12个变异类型的遗传变异进行分析,用12对AFLP引物和10个ISSR引物分别扩增出501、171个位点,多态位点百分率分别为73.6%(367)、78.8%(137)。2种标记均揭示簕竹属4个竹种间存在较大的遗传变异,但同一竹种不同变异类型间的遗传差异极小。利用AFLP,ISSR和SRAP分子标记分析了刚竹属和矢竹属4个竹种的12个变异变型的遗传变异,也得到了类似结果。Such molecular markers as amplified fragment length polymorphisms（AFLP） and inter-simple sequence repeats（ISSR） were used to analyze genetic variations among 12 infra-species of 4 species of Bambusa,with 501 and 171 bands generated using 12 AFLP primer pairs and 10 ISSR primers,respectively,of which polymorphic loci accounted for 73.6%（367 loci） and 78.8%（137）,respectively.It was revealed by two markers that there were abundant genetic variations among different bamboo species but few genetic variations among different infra-species.Similar results were obtained by AFLP,ISSR and sequence-related amplified polymorphism（SRAP） analysis of 12 infra-species of 4 species of Phyllostachys and Pseudosasa.

关 键 词：竹子 变异类型 分子标记 遗传变异 

分 类 号：S718.3[农业科学—林学]