金樱叶总皂苷的提取纯化工艺研究  

Study on Extracting and Purification Process of Total Saponin from Cherokee Rose's Leaves

作　　者：陈英文[1] 黄明宏[1] 

机构地区：[1]广州中医药大学第一附属医院,广州510405

出　　处：《光明中医》2011年第2期229-231,共3页GUANGMING JOURNAL OF CHINESE MEDICINE

摘　　要：目的优选金樱叶总皂苷的提取、纯化工艺条件。方法以齐墩果酸含量为指标,采用L9(34)正交试验设计,优选金樱叶提取工艺条件;以金樱叶总皂苷洗脱率为指标考察大孔树脂精制工艺条件。结果最佳提取工艺为60倍量70%的乙醇,50℃下提取2h,采用D-101型大孔树脂,以体积分数为40%的乙醇为洗脱剂,4倍树脂体积洗脱。结论优选工艺可较好提取纯化金樱叶总皂苷。Objective To optimize the technology parameters of extraction and purification process of total saponins from Cherokee Rose＇s Leaves.Methods With the contents of oleanolic acid,the orthogonal design L9（34 ） was adopted to optimize the extraction process.The purification process was studied by optimizing rate of total saponins as the marker.Results The optimum process was as follows,using 60 times volume of 70% ethanol extracting for 2 hours,the extraction temperature was 50℃ .D-101 macroporous resin was selected,and the eluant was 4 BV 40% ethanol.Couclusion The optimal conditions of extracting and purifying the total saponins from Cherokee Rose＇s Leaves is feasible.

关 键 词：金樱叶 总皂苷 提取 纯化 大孔树脂 实验研究 

分 类 号：R284[医药卫生—中药学]