口炎清颗粒指纹图谱研究  

The Fingerprint of Kouyanqing Granule

作　　者：关倩怡[1] 黄琳[2] 彭维[1] 王德勤[2] 苏薇薇[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]广州白云山和记黄埔中药有限公司,广东广州510515

出　　处：《中山大学学报（自然科学版）》2011年第1期115-118,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省科技计划资助项目(2010B050100013)

摘　　要：采用HPLC法构建了口炎清颗粒的指纹图谱,用Dikma PLATISIL ODS色谱柱,以乙腈-φ=0.1%甲酸溶液为流动相,检测波长为254 nm。该指纹图谱有23个共有特征峰,确认了其中7个色谱峰的成分分别为绿原酸、咖啡酸、木犀草苷、哈巴俄苷、肉桂酸、甘草酸铵、甘草苷。构建的指纹图谱,操作简便,专属性强、重复性好,为该产品的质量监控提供了有效手段。In this paper,the HPLC fingerprint of Kouyanqing Granule was established with Dikma PLATISIL ODS（250 mm×4.6 mm,5 μm） column,acetonitrile and 0.1%（φ） formic acid as mobile phase,and the detection wavelength was 254 nm.23 common peaks were selected as the fingerprint peaks.Seven of these common peaks were identified as chlorogenic acid,caffeic acid,luteolin-7-O-glucoside,harpagoside,cinnamic acid,ammonium glycyrrhetate and glycyrrhizic acid.The method established is selective,simple and reproducible,which can be used for the quality control of Kouyanqing Granule.

关 键 词：口炎清颗粒 高效液相色谱 指纹图谱 

分 类 号：R917[医药卫生—药物分析学]