辛伐他汀对实验性兔动脉粥样硬化进程中HO/CO系统的影响  

Effects of simvastatin on heme oxygenase/carbon monoxide system in atherosclerosis model rabbit

作　　者：于清霞[1] 戴琳[2] 王辉[1] 周少玲[1] 

机构地区：[1]山东省烟台毓璜顶医院,山东烟台264000 [2]山东省烟台市烟台山医院,山东烟台264001

出　　处：《实用临床医药杂志》2011年第1期58-61,共4页Journal of Clinical Medicine in Practice

摘　　要：目的探讨血红素加氧酶(HO)一氧化碳(CO)系统在动脉粥样硬化中的变化、相互关系及辛伐他汀对动脉粥样硬化进程中血红素加氧酶-一氧化碳的影响。方法家兔予以高胆固醇饮食(n=16)8周,8周后改用普通饮食并随机分为3组,模型组停用高胆固醇饮食,改普通饮食(n=8);辛伐他汀组给予喂饲辛伐他汀进行药物干预8周。同时设正常对照组(n=8):给予普通饲料喂养16周。然后取静脉血分别用Chalmers A.H、硝酸还原酶法测定各实验组血中CO,取主动脉组织用免疫组织化学方法测定主动脉组织中HO-1的表达;并比较组间各项参数的差异。结果与对照组比较,模型组血脂水平明显升高,血浆一氧化碳水平明显升高,血红素加氧酶-1表达明显升高(P均<0.01)。与模型组比较辛伐他汀组血浆TC、TG、LDL下降明显(P<0.01),HDL升高(P<0.01);血浆一氧化碳水平明显降低(P<0.01),血红素加氧酶-1表达明显减少(P<0.01)。结论动脉粥样硬化进程中,辛伐他汀可以通过下调血红素加氧酶/一氧化碳系统而延缓动脉粥样硬化进程。Objective To investigate the changes and correlation of heme oxygenase-1（HO-1）/carbon monoxide（CO） and the influence of simvastatin in atherosclerotic progress.Methods The rabbits received 1% cholesterol diet（n=16） for eight weeks;eight weeks later they were fed with normal diet for eight weeks;model group（n=8） were administrated with normol;simvastatin group（n=8） were administrated with simvastatin.The levels of serum lipids and plasma carbon monoxide were obtained at the beginning,the 8th week,and the 16th week.The expression of heme oxygenase-1 in the thoracia aortic tissue was observed by using immunohistochemistry technique.Results Compared with control group,in model group the levels of serum lipids and plasma carbon monoxide were obviously increased（P0.01）;however the expression of heme oxygenase-1 were markedly decreased（P0.01）.Compared with model group,in simvastatin group the levels of serum lipids and plasma carbon monoxide were significantly decreased（P0.01）,while the expression of heme oxygenase-1 was greatly reduced（P0.01）.Conclusions In atherosclerotic progress,heme oxygenase-1（HO-1）/carbon monoxide（CO） appears to have reciprocal relationship,and simvastatin may defer atherosclerotic progress by regulating theysystem.

关 键 词：动脉粥样硬化 血红素加氧酶 一氧化碳 辛伐他汀 免疫组织化学 

分 类 号：R543[医药卫生—心血管疾病]