糖尿病大鼠胰腺Wnt/β-catenin信号通路的变化及其调节胰岛干细胞分化与增生的意义  

The change of Wnt/β-catenin pathway in diabetic rat pancrease and its role in regulating the differentiation and proliferation of pancreatic stem cells

作　　者：杨开明[1] 胡晓松[2] 羊惠君[2] 李爱冬[2] 李茂新[1] 

机构地区：[1]大理学院基础医学院组织学与胚胎学教研室,云南大理671000 [2]成都医学院

出　　处：《中国糖尿病杂志》2011年第2期149-151,共3页Chinese Journal of Diabetes

基　　金：大理学院科研启动基金(KY421540)

摘　　要：目的研究胰岛β细胞损伤后胰岛再生过程中Wnt/β-catenin信号通路的生物调控。方法 STZ破坏新生大鼠胰岛β细胞,免疫组织化学观察1 d、2 d、3 d中Wnt/β-catenin通路β-catenin为核心的上、下游基因APC、c-Myc以及Nestin在胰岛再生过程中的表达。结果β-catenin在残留的胰岛细胞中表达上调,并在胰腺间质中异常表达;APC表达于胰腺导管上皮及残留的胰岛细胞;c-Myc则主要表达于胰腺腺泡上皮及胰腺间质内;Nestin表达分布于残留的胰岛细胞和胰腺导管上皮。结论 Wnt/β-catenin信号通路在胰岛再生中可能通过促进胰腺干细胞分化与增殖而调控。Objective To investigate the regulation role of Wnt/β-catenin signal pathway in regulating pancreatic islets regeneration after β-cells injury. Method STZ were used to injure β-cells and imunohistochemistry method was adopted to check the expression of several vital factors e.g. APC, β- catenin, c-Myc for Wnt/β-catenin signal pathway and of nestin in pancreatic islets regeneration. Results Immunohistochemistry showed that the β-catenin was up-regulated in β-cells and abnormally expressed in pancreatic stroma; APC was expressed on duct epithelium and β cells; C-myc was expressed in stroma and gland alveolus epithelium; nestin was expressed on islet cell and duct epithelium. Conclusion The regulation roles of Wnt/β-catenin signal pathway in pancreatic islets regeneration may be through promoting pancreatic stem cells differentiation and proliferation.

关 键 词：Β细胞 WNT信号 胰腺干细胞 胰岛再生 

分 类 号：R587.1[医药卫生—内分泌]