HPLC测定荆芥内酯的含量和有关物质  

Determination of Content and Related Substances in Schizonepetin by HPLC

作　　者：孙熠[1] 耿婷[1] 单鸣秋[1] 张丽[1] 丁安伟[1,2] 

机构地区：[1]南京中医药大学,南京210046 [2]江苏省方剂研究重点实验室,南京210046

出　　处：《中国实验方剂学杂志》2011年第5期52-55,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技重大专项课题:创新药物研究开发-候选药物研究(2009ZX09103-339)

摘　　要：目的:建立荆芥内酯的含量测定方法和有关物质的分析方法。方法:指定以Waters XTerra MS C18色谱柱进行有关物质分析,指定以Kromasil C18色谱柱进行含量测定,以甲醇-水(45∶55)为流动相(氢氧化钠调pH至10.0),流速为0.8mL·min-1,柱温30℃,检测波长216 nm。结果:荆芥内酯与有关物质及各降解成分完全分离;荆芥内酯质量浓度在0.012 6～0.403 6 g·L-1与峰面积呈良好线性关系,r=0.999 9;最低检测限0.25μg,最小定量限为0.63μg;平均回收率100.04%,RSD为1.40%。结论:该方法操作简便、快速、准确,灵敏度高,可用于荆芥内酯含量及有关物质的测定。Objective：To establish an HPLC method for the determination of content and related substances in schizonepetin. Method：Two stability columns based on silica gel were used respectively（ Waters XTerra MS C18 column for related substances analysis; And Kromasil C18 column for content determination）. The mobile phase con- sisted of methanol-water（45：55 ）, and when analyzing substances destroyed by alkali, the pH value of the mobile phase should be adjusted to 10.0 by sodium hydroxide. Both of the two columns were maintained at a constant tem- perature of about 30 ℃. The flow rate was 0.8 mL per minute. The UV detection wavelength was set at 216 nm. Resuit：Related substances and degraded substances were completely separated from schizonepetin. The linear range of determination was 0. 012 6-0. 403 6 g.L-1 with the correlation coefficient of 0. 999 9. The LOD was 0.25 μg,and the LOQ was 0.63 μg. The average recovery was 100.04% , RSD 1.40%. Conclusion： The method is simple, rapid, accurate, and sensitive and it is suitable for the determination of the content and related substances in schizonepetin.

关 键 词：荆芥内酯 高效液相色谱法 含量 有关物质 

分 类 号：R284.1[医药卫生—中药学]