酸枣仁合欢方中酸枣仁皂苷A的薄层色谱鉴别和含量测定  

Identification and Determination of Jujuboside A in Suanzaoren Hehuan Formula

作　　者：刘婧姝[1] 乔卫[1] 郝兰芳[1] 李陆[1] 杨奕[1] 

机构地区：[1]天津医科大学药学院,天津300070

出　　处：《中国实验方剂学杂志》2011年第5期77-79,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：天津市卫生局基金资助项目(02020)

摘　　要：目的:建立中药复方酸枣仁合欢方中酸枣仁皂苷A的定性鉴别以及高效液相色谱-蒸发光散射检测法的含量测定方法。方法:采用薄层色谱法对方剂中的有效成分酸枣仁皂苷A进行鉴别。采用Ameritech Accurasil C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(60∶40),流速1.0 mL·min-1,柱温35℃,检测器漂移管温度102℃。结果:在薄层色谱图谱中可以检出酸枣仁皂苷A,斑点清晰可见,重复性好。酸枣仁皂苷A检测浓度在0.6～3.0μg与峰面积呈良好的线形关系(r=0.999 9),平均回收率为100.16%(RSD 1.63%)。结论:定性定量方法简便、准确、重复性好,可作为酸枣仁合欢方的质量控制方法。Objective ： To establish a TLC method for identification of Semen Zizyphi Spinosae and a HPLC- ELSD method for determination of jujuboside A in Suanzaoren Hehuan Formula. Method ： Quality of Semen Zizyphi Spinosae was identified with thin layer chromatography. The analytical column was Ameriteeh Aceurasil-ODS （4.6 mm × 250 mm ,5 μm）. The mobile phrase consist of methanol-water（60： 40）. The flow rate was 1.0 mL- min - 1. The column temperature was set at 35 ℃. The tube temperature of the detector was 102 ℃. Result：TLC could be used for the identification of Semen Zizyphi Spinosae,the spots were clear and accurate. The jujuboside A was linear within the range of 0.6-3.0 μg（r = 0. 999 9）. The average recovery was 99.98% and RSD was 1. 19%. Conclusion： These methods are sensitive, accurate and simple. The methods can be used for the quality control of Suanzaoren Hehuan Formula.

关 键 词：酸枣仁合欢方 薄层色谱法 高效液相色谱一蒸发光散射检测法 含量测定 

分 类 号：R284.1[医药卫生—中药学]