左归降糖清脂方对2型糖尿病转基因MKR鼠脂肪肝发生的影响  

Influences of Zuogui Jiangtang Qingzhi Recipe on Liver Steatosis in Type 2 Diabetes MKR Mice

作　　者：吴勇军[1] 成细华[1] 喻嵘[1] 夏金华[1] 伍参荣[1] 

机构地区：[1]湖南中医药大学,长沙410007

出　　处：《中国实验方剂学杂志》2011年第5期140-143,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30973749);湖南省自然科学基金杰出青年基金(08JJ1005);湖南省中医药科研计划项目(2009017);湖南省科技厅资助项目(2009SK3118);湖南省高校创新平台开放基金(09K059);湖南中医药大学方剂学重点学科资助

摘　　要：目的:探讨左归降糖清脂方对2型糖尿病转基因MKR鼠脂肪肝发生的影响。方法:30只MKR鼠随机分为MKR组、MKR高脂组和左归降糖清脂方组;左归降糖清脂方组以生药29.64 g.kg-1 ig干预治疗高脂饮食诱发脂肪肝的MKR鼠,观察其肝脏病理形态、血糖、血胰岛素、肝功能和血脂变化。结果:高脂饲料诱发MKR鼠形成非酒精性脂肪肝,肝细胞有大小不一的脂滴,呈脂肪性变;MKR高脂组小鼠空腹血糖、血胰岛素、肝质量、肝指数、肝功能指标[谷丙转氨酶(ALT),谷草转氨酶(AST)]、血清总胆固醇(TCHO)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)高于MKR组(P<0.05,P<0.01),甘油三酯(TG)下降,低于MKR组(P<0.01)。2型糖尿病并发脂肪肝MKR鼠经左归降糖清脂方治疗后,肝细胞内脂肪性变得到改善,空腹血糖、血胰岛素、肝质量、肝指数、肝功能指标(ALT,AST)和血脂(TCHO,LDL-C和TG)显著下降,低于MKR小鼠(P<0.05,P<0.01);HDL-C高于MKR小鼠(P<0.05)。结论:左归降糖清脂方可通过调节高脂饮食MKR鼠体内的糖脂代谢,改善肝脏脂肪性变。Objective：To observe the protective effect of Zuogui Jiangtang Qingzhi Recipe（ZJQR） on the liver steatosis in type 2 diabetes MKR mice.Method：Thirty MKR mice were randomly divided into the control group（CG）,the model group（MG） and the ZJQR group（ZG）.Except for CG mice which were raised by standard animal feeds,the others were all raised by high fat feeds to make liver steatosis model and after 8 weeks ZG were adminis-tered by ZJQR for 4 week.Histopathological changes were observed in the liver,including fasting blood-glucose（FBG）,insulin（INS） level,blood-lipid,liver quality,hepatosomatic index and liver function.Result：In the MG,the histopathological investigation showed increased fatty droplet within hepatocyte and fatty degeneration.As were liver quality,hepatosomatic index,the levels of FBG,INS,total cholesterol（TCHO）,high density lipoprotein cholesterol（HDL-C） and low-density lipoprotein cholesterol（LDL-C）（P 〈 0.05,P 〈 0.01）,but the level of triglycercide（TG） was reduced compared with CG（P 〈 0.01）.In the ZG,fatty degeneration within hepatocyte was improved·Liverquality,hepatosomatic index,the levels of FBG,INS,TCHO,LDL-C and TG were reduced（P 〈 0.05）,but HDL-C was enhanced compared with that in CG（P 〈 0.05,0.01）.Conclusion：ZGQR can significantly regulate glucose-lip-id metabolism,improve fatty degeneration and protect the liver.

关 键 词：左归降糖清脂方 2型糖尿病 MKR鼠 非酒精性脂肪肝 血脂 肝功能 

分 类 号：R285.5[医药卫生—中药学]