祁州漏芦对H22小鼠肝癌皮下移植瘤的抑瘤作用及其机制初探  

Studies on Anti-tumor Effect and Mechanism of Rhaponticum uniflorum in H22-bearing Mice

作　　者：金爱花[1] 许惠仙[2] 刘文静[1] 全吉淑[1] 尹学哲[2] 

机构地区：[1]延边大学基础医学院,吉林延吉133000 [2]延边大学附属医院,吉林延吉133000

出　　处：《中国实验方剂学杂志》2011年第5期165-167,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省科技发展计划资助项目(200705428);吉林省中医药管理局科技项目(08SYS-105)

摘　　要：目的:探讨祁州漏芦水提物(RUWE)对小鼠H22移植瘤的生长抑制作用。方法:建立小鼠皮下H22移植瘤模型,将实验动物分为模型组、RUWE高、中、低剂量组和5-Fu组,RUWE按400,200,100 mg.kg-1.d-1剂量ig连续给药10 d,5-Fu组按25 mg.kg-1.d-1剂量隔日ip给药,处死动物,计算抑瘤率、胸腺指数和脾指数,并以ELISA法检测血清肿瘤坏死因子-α(TNF-α)和白介素-2(IL-2),比色法检测血清总抗氧化活力(T-AOC)和丙二醛(MDA)含量。结果:与模型组相比,RUWE能显著减小移植瘤瘤重,提高抑瘤率,其RUWE高、中、低剂量组抑癌率分别为38.05%,34.98%,26.95%。同时,明显升高荷瘤小鼠的脾指数,增高血清IL-2和降低TNF-α,增高血清T-AOC和降低MDA水平。结论:RUWE对H22移植瘤具有明显的抑制作用,其作用可能与其增高机体免疫机能和抗氧化能力有关。Objective：To investigate the anti-tumor effect of Rhaponticum uniflorum water extract（RUWE）in H22-bearing mice.Method：The H22 transplanted mouse models were established and randomly divided into 5 groups：model,high,medium and low dose RUWE,and 5-Fu group.Animals were treated with ig 400,200,100 mg.kg-1.d-1 RUWE once daily for 10 days,and with ip 5-Fu 25 mg.kg-1.d-1 every other day for 3 times,then the tumor growth in-hibition ratios,spleen and thymus indexes were caluclated.The serum tumor necrosis factor-α（TNF-α）and interlekin-2（IL-2）was determined with ELISA method,the total antioxidant capacity（T-AOC）and malondialdehyde（MDA）con-tent were detected by colorimetric method.Result：The administration with 400,200,100 mg.kg-1.d-1 RUWE inhibi-ted the growth of transplanted tumor with the inhibitory ratios of 38.05%,34.98%,26.95%,respectively.In addi-tion,RUWE treatment elevated the spleen index,reduced the serum TNF-α level,increased the serum T-AOC and de-creased serum MDA level of H22-bearing mice.Conclusion：RUWE could exhibit an inhibitory effect on the growth of transplanted H22 tumor,probably via the improved immune function and antioxdative capability.

关 键 词：漏芦 H22 抗肿瘤 抗氧化 

分 类 号：R285.5[医药卫生—中药学]