补肾醒脑方对血管性痴呆大鼠血管内皮生长因子、白介素-1β和肿瘤坏死因子-α表达的影响  

Upregulation of Vascular Endothelial Growth Factor and Downregulation of IL-1β,Tumor Necrosis Factor-α by Bushen Xingnao Decoction in Vascular Dementia Rats

作　　者：胡久略[1,2] 贺又舜[2] 张超云[1] 赵清超[1] 

机构地区：[1]南阳理工学院,河南南阳473004 [2]湖南中医药大学,长沙410007

出　　处：《中国实验方剂学杂志》2011年第5期190-193,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：南阳市科技公关计划项目(2007S1408)

摘　　要：目的:观察补肾醒脑方对血管性痴呆大鼠脑组织和血清血管内皮生长因子(VEGF)、白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)表达的影响,探讨补肾醒脑方对血管性痴呆脑保护作用机制。方法:Wistar大鼠随机分组:正常组(N组)、假手术组(S组)、痴呆模型组(M组)、补肾醒脑方治疗组(MT组)。选取造模成功后2,4,6周为观察点,用酶联免疫吸附法检测大鼠脑组织和血清VEGF,IL-1β,TNF-α表达水平。结果:MT组IL-1β,TNF-α表达较M组降低(P<0.05),MT组VEGF表达较M组增高(P<0.05);MT组VEGF,IL-1β,TNF-α表达较N,S组增高(P<0.05);M组VEGF,IL-1β,TNF-α表达较N,S组显著增高(P<0.01)。结论:补肾醒脑方能降低血管性痴呆大鼠脑组织和血清IL-1β,TNF-α水平,增强VEGF表达,补肾醒脑方可以通过抑制血管性痴呆大鼠神经炎性反应和增强血管修复发挥脑保护作用。Objective：To investigate influences of Bushen Xingnao Decoction（BSXND）on expression of vas-cular endothelial growth factor（VEGF）,IL-1β and tumor necrosis factor-α（TNF-α） in vascular dementia brain and se-rum level of VEGF,IL-1β and TNF-α in vascular dementia rats,to study neuroprotective mechanism of BSXND for vas-cular dementia.Method：Wistar rats were randomly divided into：normal group（N）,sham group（S）,vascular dementia model group（M）and minocycline treatment group（MT）.After the model was successfully established,observation was carried out at 2,4,6 weeks.Expression of VEGF,IL-1β and TNF-α in serum and brain were measured by enzyme linked immunosorbent assay.Result：The expressions of IL-1β and TNF-α in MT groups were lower than that of in M（P 〈0.05）,the expression of VEGF in MT groups was higher than that of in M（P 〈0.05）;the level and expression of VEGF,IL-1β and TNF-α in MT and M groups were higher than that in N and in S（P 〈 0.01）.Conclusion：BSXND can restrain the expression of IL-1β,TNF-α and increase the expression of VEGF in vascular dementia.BSXND can enhance recovery of cerebral vascular to protect neural function by restraining neuroinflammation.

关 键 词：血管性痴呆 补肾醒脑方 血管内皮生长因子 肿瘤坏死因子-Α 白介素-1Β 

分 类 号：R285.5[医药卫生—中药学]