人源脑红蛋白转基因秀丽线虫的制备、鉴定及生理功能分析  

Construction,characterization and physiological functions of transgenic C.elegans expressing human neuroglobin

作　　者：石锦平 任长虹[2] 李媛[2] 张继业[2] 范礼斌[1] 张成岗 

机构地区：[1]安徽医科大学生命科学学院生物学教研室,安徽合肥230032 [2]军事医学科学院放射与辐射医学研究所蛋白质组学国家重点实验室,北京100850

出　　处：《军事医学》2011年第1期21-24,43,共5页Military Medical Sciences

基　　金：国家973计划项目(2006CB504100);国家科技重大专项课题(2009ZX09103-616;2009ZX09301-002;2009ZX09503-002);国家自然科学基金项目(30771230;30600365);中国博士后科学基金特别资助基金(200801495)

摘　　要：目的以秀丽隐杆线虫为模式生物制备人源脑红蛋白(human neuroglobin,hNgb)转基因线虫,初步探讨外源性hNgb对线虫寿命和产卵率的影响。方法通过显微注射方法制备hNgb转基因线虫,使用蛋白印迹和免疫组织化学进行鉴定。使用野生型N2和增强型绿色荧光蛋白(EGFP)转基因线虫作为对照组,测量转基因线虫的寿命和产卵率。结果成功获得hNgb转基因株系线虫,其寿命和产卵率与野生型N2和EGFP转基因线虫相比,差异无统计学意义。结论外源性hNgb转基因不影响线虫的发育和正常生理功能,可用于Ngb功能的深入研究。Objective To construct the transgenic Caenorhabditis elegans expressing human neuroglobin（hNgb） and to investigate the effect of hNgb on the brood size and lifespan of C.elegans.Methods The transgenic C.elegans expressing hNgb was obtained with microinjection.Expression of hNgb in the transgenic strains was detected by Western blot and immunohistochemistry.The life span and brood size of the C.elegans were detected using wild type N2,while the enhanced green fluorescin protein（EGFP） transgenic strain was used as a control.Results The hNgb transgenic strain was obtained.Compared with wild type C.elegans and EGFP transgenic strain,the transgenic hNgb C.elegans was no statistically significant difference in the life span and brood size.Conclusion The hNgb transgenic strain has no obvious effect on nematode′s normal physiological function and could be used for the further functional identification of hNgb.

关 键 词：脑红蛋白 秀丽隐杆线虫 转基因株系 产卵率 寿命 

分 类 号：R383.1[医药卫生—医学寄生虫学]