葫芦茶抗IgE介导Ⅰ型过敏有效部位的研究  

Effect of active part extracted from Tadehagi trquetrum(Linn.) Ohashi on type Ⅰ allergy induced by IgE

作　　者：周旭东[1] 史丽颖[1] 于大永[1] 师海波[2] 苗艳波[2] 唐玲[1] 冯宝民[1] 王永奇[1] 

机构地区：[1]大连大学药物研究所,辽宁大连116622 [2]吉林省中医药科学院,长春130041

出　　处：《中南药学》2011年第1期35-38,共4页Central South Pharmacy

摘　　要：目的以大鼠I型过敏反应模型探索葫芦茶抗过敏反应有效部位。方法葫芦茶用50%丙酮提取、乙酸乙酯萃取和D101大孔树脂富集分别得到丙酮提取物(1号)、乙酸乙酯萃取物(2号)、水溶物(3号)、水不溶物(4号)和大孔树脂富集物(5号)。采用卵白蛋白(OVA)和氢氧化铝[Al(OH)3]的混合液致敏和诱发制备的大鼠I型过敏反应模型,随机分组,1、2、3、4、5号组各设100、400 mg.kg-12个剂量组,孟鲁司特钠(menglus-itena)组、正常对照组和模型组;采用ELISA法测定血清IgE、LT和His含量;采用细胞计数法,测定大鼠全血和肺泡灌洗液(BALF)中嗜酸性粒细胞(EOS)数量;通过病理学检查,检测肺组织炎症面积。结果 1、2、3号均可明显降低血清IgE、LT和His的含量,强弱顺序为1>2>3;1号可明显减少全血及BALF中EOS数量,其余作用不明显;1、2号可减少肺组织炎症面积,强弱顺序为1>2。结论 1、2、3号灌胃给药对大鼠过敏均有明显的拮抗作用,综合各项指标,活性强弱顺序为1>2>3。Objective To determine the effect of active part extracted from Tadehagi trquetrum（Lim.） Ohashi on type Ⅰ allergy induced by IgE.Methods Tadehagi trquetrum（Linn.） Ohashi was extracted by 50% acetone.The 50% acetone extractions were again extracted by EtOAc and isolated by D101 chromatography column.We got 50% acetone extraction（1）,EtOAc extraction（2）,H2O layer（3）,residue（4）,and enriched extract by macroporous resin D101（5）.Rats were sensitized with the mixture of OVA and Al（OH）3 to establish the experimental rat model of type Ⅰ allergy.The rats were divided into 13 groups ramdomly,namely a normal group,a model group,a menglusitena group,and sample group 1,2,3,4,5 at 100 mg·kg-1 or 400 mg·kg-1 respectively.IgE,LT and His contents in the serum of sensitized rats were measured with ELISA.The inflammatory area in the pulmonary tissues through pathological examination.Results Group 1,2,3 decreased the contents of IgE,LT and His in the serum.The active sequence was 123.Group 1 reduced the quantities of EOS in whole blood and BALF;Group 1 and 2 reduced the inflammatory areas in the pulmonary tissue,and the active sequence was 12.Conclusion Group 1,2,and 3 have obvious anti-alergy effect on type Ⅰ allergy and the active sequence is 123.

关 键 词：葫芦茶 有效部位 IgE介导I型过敏反应 炎症 

分 类 号：R965.1[医药卫生—药理学]