The role of tocopherol cyclase in salt stress tolerance of rice(Oryza sativa)  

作　　者：OUYANG ShouQiang HE SiJie LIU Peng ZHANG WanKe ZHANG JinSong CHEN ShouYi 

机构地区：[1]State Key Laboratory of Plant Genomics and National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [2]Graduate School of the Chinese Academy of Sciences, Beijing, 100049, China

出　　处：《Science China(Life Sciences)》2011年第2期181-188,共8页中国科学（生命科学英文版）

基　　金：supported by the National Basic Research Program of China (Grant No. 2006CB100102);National High-Tech Project (Grant No. 2006AA10Z18201)

摘　　要：Tocopherols synthesized exclusively by photosynthetic organisms are major antioxidants in biomembranes.In plants,tocopherol cyclase(TC/VTE1) catalyzes the conversion of 2,3-dimethyl-5-phytyl-1,4-benzoquinone(DMPBQ) to γ-tocopherol.In the present study,OsVTE1,which encodes a rice tocopherol cyclase ortholog,was cloned and characterized.OsVTE1 was induced significantly by abiotic stresses such as high salt,H2O2,drought,cold and by the plant hormones ABA and salicylic acid.The tissue-specific expression pattern and OsVTE1-promoter GUS activity assay showed that OsVTE1 was mainly expressed in the leaf,and also could be detected in the root,stem and panicle.Compared with control plants,transgenic plants with OsVTE1 RNA interference(OsVTE1-RNAi) were more sensitive to salt stress whereas,in contrast,transgenic plants overexpressing OsVTE1(OsVTE1-OX) showed higher tolerance to salt stress.The DAB in vivo staining showed that OsVTE1-OX plants accumulated less H2O2 than did control plants.Tocopherols synthesized exclusively by photosynthetic organisms are major antioxidants in biomembranes.In plants,tocopherol cyclase（TC/VTE1） catalyzes the conversion of 2,3-dimethyl-5-phytyl-1,4-benzoquinone（DMPBQ） to γ-tocopherol.In the present study,OsVTE1,which encodes a rice tocopherol cyclase ortholog,was cloned and characterized.OsVTE1 was induced significantly by abiotic stresses such as high salt,H2O2,drought,cold and by the plant hormones ABA and salicylic acid.The tissue-specific expression pattern and OsVTE1-promoter GUS activity assay showed that OsVTE1 was mainly expressed in the leaf,and also could be detected in the root,stem and panicle.Compared with control plants,transgenic plants with OsVTE1 RNA interference（OsVTE1-RNAi） were more sensitive to salt stress whereas,in contrast,transgenic plants overexpressing OsVTE1（OsVTE1-OX） showed higher tolerance to salt stress.The DAB in vivo staining showed that OsVTE1-OX plants accumulated less H2O2 than did control plants.

关 键 词：RICE TOCOPHEROL tocopherol cyclase（TC/VTE1） transgenic rice abiotic stress tolerance 

分 类 号：Q945.78[生物学—植物学] TQ465.1[化学工程—制药化工]