S-腺苷蛋氨酸抗小鼠H22肝癌作用研究  

Anti-Tumor Effects of S-adenosyl-L-Methionine in Mouse Liver Cancer H22 Cells

作　　者：姚毅[1] 张宇[1] 张帆[1] 侯景[1] 罗新龙[1] 曹荣月[1] 金亮[1] 吴洁[1] 刘景晶[1] 

机构地区：[1]中国药科大学生命科学与技术学院微基因药物实验室,江苏南京210009

出　　处：《药物生物技术》2011年第1期43-47,共5页Pharmaceutical Biotechnology

基　　金：国家自然科学基金资助项目(项目编号:30772570;30872393);中央高校基本科研业务费专项资金资助(JKY2009021;JKQ2009022);国家基础科学人才培养基金(J0630858)

摘　　要：探讨S-腺苷蛋氨酸(SAM)能否抑制小鼠H22肝癌。通过建立H22小鼠肝癌模型,SAM预防及治疗,检测皮下抑瘤率、皮内肿瘤微血管新成、肺转移等指标对其抗肿瘤作用机理进行部分探讨。结果显示,SAM对H22肿瘤攻击起到有效地保护作用,与PBS阴性对照组相比,预防组和治疗组肿瘤生长缓慢,平均瘤重显著降低(P<0.01),抑瘤率达到70%以上;有效地抑制了小鼠皮内模型中的微血管新生(P<0.01);同时在一定程度上减少了小鼠的肺转移(P<0.05)。SAM通过抑制肿瘤血管生长表现出有效的抑制小鼠H22肝癌作用。After setting up the animal model of H22,the anti-tumor effects of S-adenosyl-L-methionine（SAM） were investigated by detecting tumor inhibitory rate,tumor-induced angiogenesis and pulmonary metastasis to explore whether SAM can effectively inhibit H22 and to study the mechanism of SAM on anti-tumor.The results of experiment showed that SAM significantly inhibited the growth of H22 in mice.The average tumor weight was significantly lower in the mice treated with SAM,than that in mice injected with PBS（P0.01）.The tumor inhibitory rate is more than 70%.Moreover,SAM attenuated tumor-induced angiogenesis in intradermal tumor model in mice（P0.01）.Meanwhile,SAM can also significantly reduce the number of pulmonary metastases in mice（P0.01）.SAM can effectively suppress the growth of H22 cancer in mice by inhibiting angiogenesis of tumor.

关 键 词：S-腺苷蛋氨酸 H22肝癌 抑制血管生成 肺转移 

分 类 号：R963[医药卫生—微生物与生化药学]