瓜蒌皮提取物对大鼠主动脉PDC Ca^(2+)内流的影响  

Influence of Extract of Mesocarp of Mongolian Snakegourd (Trichosanthes kirilowii) on Transmembrane Ca^(2+) Influx in Rat Aortic Ring

作　　者：莫尚武[1] 刘宁[1] 张坐奎[1] 吴玉蓉[2] 杨涛四 

机构地区：[1]四川大学原子核科学技术研究所,成都610064 [2]四川大学化学系 [3]川大学化学系

出　　处：《中草药》1999年第9期674-676,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金!19775034;核工业科学基金

摘　　要：为了研究瓜蒌皮治疗冠心病的有效成分，用醚醇法和水煎醇沉法提取瓜蒌皮的有效成分，并用^(４５)Ｃａ跨膜测量方法研究各提取物对大鼠主动脉ＰＤＣ钙通道Ｃａ^(２＋)内流的影响。发现氯仿和醇溶物能阻滞Ｃａ^(２＋)内流，水溶物具有相反的作用，其中氯仿提取物浓度为３０ ｍｇ／ｍＬ时，使Ｃａ^(２＋)内流量比对照下降３１．５７％。实验结果表明瓜蒌皮中可能有７种组分具有治疗冠心病的活性。Powdered mesocarp of Trichosanthes kirilowii Maxim. was extracted with petroleum ether, ethanol and water, partitioned with chloroform and ethyl acetate or precipitated with the addition of ethanol to obtain a total of 14 fractions. Their effects on Ca^(2+) influx of rat aortic rings were studied with ^(45)Ca transmembrane influx technique. Results showed that 7 fractions could block potential dependent Ca^(2+) influx, suggesting their potential use for the treatment of coronary heart diseases. Among them, chloroform extract resulted in a decrease of Ca^(2+) influx of 31.57 % in comparison with the control.

关 键 词：瓜蒌皮 钙拮抗剂 放射性同位素 冠心病 钙^45 

分 类 号：R285.5[医药卫生—中药学] R286.2[医药卫生—中医学]