镉处理下不同富集镉花生营养器官AhMTⅡ mRNA表达变化  

AhMTⅡ mRNA Expression Changes of Peanut Nutritorium (Arachis hypogaea L.) with Different Cadmium Absorption under Cadmium Treatment

作　　者：李春娟[1] 单世华[1] 杨志艺[1] 闫彩霞[1] 张廷婷[1] 范仲学[2] 万书波[2] 

机构地区：[1]山东省花生研究所,山东青岛266100 [2]山东省农业科学院,济南250100

出　　处：《中国农业科技导报》2011年第1期20-24,共5页Journal of Agricultural Science and Technology

基　　金："十一五"国家科技支撑计划项目(2006BAD21B04);国家自然科学基金项目(30771361);现代农业产业技术体系建设专项资金项目资助

摘　　要：利用含镉（Cd2＋）营养液培养花生幼苗,取不同营养器官用以检测AhMTⅡmRNA表达量的变化。构建了PMD18T-AhMTⅡ和PMD18T-actin重组质粒,用SYBR GreenⅠ实时定量PCR方法精确分析了花生AhMTⅡmRNA表达水平。研究结果表明,花生幼苗期不同营养器官间AhMTⅡmRNA表达量差异显著（P〈0.01）：根〉叶〉茎,低富集镉花生品种XD1011根部AhMTⅡmRNA表达量显著低于高富集镉花生品种XA004。AhMTⅡmRNA表达与镉处理水平具有高度相关性,尤其是根部和叶部,在0~50μmol/L镉处理水平间,品种XD1011和品种XA004根部相关系数分别为0.977和0.96,品种XD1011叶部相关系数为0.91,而品种XA004在0~200μmol/L镉处理水平间相关系数为0.959。该结果可为筛选低富集镉花生品种提供理论与技术支持。Different vegetative organs of peanut seedlings cultured with nutrient solution containing Cd2＋ were collected for testing the changes of metallothionein type Ⅱ（AhMTII） mRNA level.PMD18T-AhMTⅡ and PMD18T-actin plasmids were constructed,and then AhMTⅡ mRNA level was analyzed using SYBR Green I real-time quantitative PCR.The result indicated that the AhMTⅡ mRNA level of different tissues showed significant（P0.01） difference： root leaf stem,and the AhMTⅡ mRNA content in root of low cadmium accumulation peanut variety XD1011 was obviously lower than that in cadmium accumulation peanut variety XA004.AhMTⅡ mRNA expression had high correlation with cadmium levels,especially in the roots and leaves.Under 0~50 μmol/L cadmium levels,the R2 of XA004 and XD1011′s roots were 0.977 and 0.96,respectively and the R2 of XD1011′s leaf is 0.91;the R2 of XA004 in leaf is 0.959 under 0~200 μmol/L cadmium level.The above results would provide theoretical and technical support for screening peanut varieties with low cadmium absorption.

关 键 词：实时定量PCR 花生 AhMTⅡ 镉 

分 类 号：S565.2[农业科学—作物学] Q786[生物学—分子生物学]