应用弹性蛋白酶复制金黄地鼠肺气肿模型  

Inducement of Emphysematous Model Using Elastase in Hamsters

作　　者：张旭晨[1] 阮英茆[1] 徐新林[1] 褚燕 韩晓男[1] 李亚辉[1] 

机构地区：[1]中国医学科学院阜外心血管病医院中国协和医科大学心血管病研究所,北京100037

出　　处：《天津医药》1999年第9期536-538,共3页Tianjin Medical Journal

基　　金：家"九五"攻关资助

摘　　要：应用弹性蛋白酶气管内滴入复制金黄地鼠肺气肿模型。方法：将金黄地鼠麻醉后，气管内一次性滴入弹性蛋白酶液（４０ ｕ／１００ ｇ体重），分别于实验第 ３天、１周、２周、３周、４周时处死。对地鼠肺脏进行病理学检查，测定第４周时平均肺动脉压、右室收缩压、右心室肥大指数、肺容积。结果：实验第４周时，地鼠出现了典型的肺气肿改变，平均肺动脉压、右室收缩压、右心室肥大指数、肺容积均明显高于对照组（Ｐ＜０．０５或Ｐ＜０．０１）。结论：气管内一次性滴入弹性蛋白酶可成功地复制出金黄地鼠肺气肿模型。Objective: To develop an emphysematous model in hamsters using porcine pancreatic elastase(PPE) intratracheal admin istration. Methods: Emphysema was induced in hamsters using PPE(40 unit/100 g body weight), the pathological changes were observed at 3d, 1w, 2w, 3w, 4w after intratracheal administration of PPE, and the mean pulmonary artery pressure (MPAP), right ventricular systolic pressure(RVSP), ration of right ventricular hypertrophy(RVHR), volume of lung(LV) were measured at 4 weeks after intratracheal administration of PPE. Results: Typical emphysematous changes occurred, and the MPAP,RVSP, RVHR, LV were increased significantly in hamsters with administration of PPE than these of control hamsters at 4 weeks. Conclusion: Intratracheal administration of PPE may successfully induce emphysema in hamsters.

关 键 词：肺气肿 胰肽酶 动物实验 疾病模型 

分 类 号：R563.302[医药卫生—呼吸系统]