甲氰咪呱对鼠体内IL-2/LAK抗肿瘤作用的影响  

Influence of Cimetidine on IL 2/LAK Antitumor Effects of Mice In Vivo

作　　者：张明智[1] 张中冕[1] 王瑞林 杜献堂 赵曼瑞 

机构地区：[1]河南医科大学一附院肿瘤科 [2]河南医科大学二附院肿瘤科

出　　处：《实用癌症杂志》1999年第3期171-173,共3页The Practical Journal of Cancer

摘　　要：为探讨甲氰咪呱（ＣＩＭ）对鼠体内ＩＬ２／ＬＡＫ抗肿瘤作用的影响，将Ｂ１６细胞经尾静脉接种于Ｃ５７ＢＬ／６小鼠，第３ｄ分别应用ＤＨａｎｋｓ液、ＣＩＭ（１０ｍｇ／ｍｌ）＋ＣＩＭ（１μｇ／ｍｌ）诱导的脾细胞，ＩＬ２＋ＩＬ２（５００Ｕ／ｍｌ）诱导的ＬＡＫ细胞，ＣＩＭ（１０ｍｇ／ｍｌ）＋ＩＬ２＋ＣＩＭ（１μｇ／ｍｌ）和ＩＬ２（５００Ｕ／ｍｌ）共同诱导的ＬＡＫ细胞，观察鼠肺转移结节和生存期。结果显示，单用ＣＩＭ并不能减少肺转移结节和延长生存期；ＣＩＭ＋ＩＬ２／ＬＡＫ治疗组与ＩＬ２／ＬＡＫ治疗组相比，可减少肺转移结节（Ｐ＜０．０５）和显著延长生存期（Ｐ＜０．０１）。结果表明，ＣＩＭ能够增强ＩＬ２／ＬＡＫ抗肿瘤作用，可用于治疗肿瘤。To investigate the influence of cimetidine(CIM) on IL 2/LAK antitumor effects of mice in vivo,C57BL/6 mice were injected intravenously with B16 cells and on the third day treated with:D Hanks;CIM(10 mg/ml)+splenocytes induced by CIM(1 μg/ml)(CIM);IL 2+syngeneic LAK cell induced by IL 2(500 U/ml)(IL 2/LAK);CIM(10 mg/ml)+IL 2+syngeneic LAK cell induced by CIM(1 μg/ml) and IL 2(500 U/ml)(CIM+IL 2/LAK) respectively.The number of pulmonary metastases was counted and survival time was observed.Results:The number of pulmonary metastases of mice was not reduced and survival time was not prolonged by CIM alone(both P >0.05);The number of pulmonary metastases of mice was reduced( P <0.05) and survival time was significantly prolonged( P <0.01) by CIM+IL 2/LAK compared with IL 2/LAK alone.Results indicated that CIM could augment antitumor effects of IL 2/LAK.

关 键 词：甲氰咪呱 IL-2 LAK 抗肿瘤作用 免疫增强 

分 类 号：R975[医药卫生—药品] R979.5[医药卫生—药学]