缺氧对滋养细胞E-钙黏蛋白表达的影响  

Effect of hypoxia on expression of E-cadherin in trophoblast cells

作　　者：余俊[1] 乔福元[1] 冯玲[1] 刘海意[1] 周琼[1] 吴媛媛[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,湖北武汉430030

出　　处：《中国妇幼保健》2011年第4期581-583,共3页Maternal and Child Health Care of China

摘　　要：目的:了解在缺氧条件下,单层贴壁培养的滋养细胞系TEV-1中,E-钙黏蛋白(E-cadherin,E-cad)的表达在mRNA水平和蛋白水平变化情况,探讨缺氧环境对滋养细胞迁移能力的影响,深入了解缺氧环境诱发子痫前期发病的潜在分子机制。方法:体外贴壁培养的滋养细胞系(TEV-1),分别直接接种于24孔板进行细胞爬片和培养瓶内,分为常氧组和缺氧组。常氧组加新鲜DMEM/F12培养基,缺氧组加含终浓度为300μmol/L二氯化钴的培养基,然后在正常条件下(37℃,5%CO2)培养。两组细胞培养48 h后,分别收集细胞,采用Trizol法抽提mRNA,并进行RT-PCR,检测E-钙黏蛋白的mRNA表达水平;细胞爬片采用免疫组织化学法进行染色,检测E-钙黏蛋白的蛋白表达水平。统计数据分析两组差异性。结果:二氯化钴化学缺氧条件下培养后,滋养细胞E-钙黏蛋白的mRNA和蛋白表达水平均较常氧对照组升高,差异具有统计学意义(P<0.05)。结论:缺氧环境可能导致滋养细胞E-钙黏蛋白的mRNA和蛋白的表达水平升高,由此降低了滋养细胞的迁移能力。这可能是缺氧造成胎盘浅着床、继而发生子痫前期的重要分子机制。Objective：To understand the changes of E-cadherin mRNA and protein expressions in trophoblast cells line TEV-1 cultured in vitro adherently on the condition of hypoxia,explore the effect of hypoxia on migration of trophoblast cells,understand the potential molecular mechanism of onset of preeclampsia induced by hypoxia.Methods：The trophoblast cells line（TEV-1） cultured in vitro adherently were inoculated into 24 orifice plate and culture bottle,and divided into normoxic group and hypoxic group;the trophoblast cells line in normoxic group was cultured with fresh DMEM/F12 culture medium,and the trophoblast cells line in hypoxic group was cultured with culture medium containing 300 μmol/L cobaltous chloride（terminal concentration）,the culture conditions were 37 ℃ and 5%CO2;after 48 hours,the cells were collected,Trizol method was used to extract mRNA,then RT-PCR was carried out,the expression level of E-cadherin mRNA was detected;immunohistochemical staining was used in the study,the expression level of E-cadherin protein was detected;statistical differences were analyzed.Results：The expression levels of E-cadherin mRNA and protein in hypoxic group were significantly higher than those in normoxic group（P〈0.05）.Conclusion：Hypoxia may induce the increases of expression levels of E-cadherin mRNA and protein in trophoblast cells,and reduce the migration ability of trophoblast cells,which may be an important molecular mechanism of shallow implantation of placenta and occurrence of preeclampsia.

关 键 词：滋养细胞 E-钙黏蛋白 缺氧 

分 类 号：R714.56[医药卫生—妇产科学]