大鼠自体原位肝移植胆道缺血再灌注损伤胆道组织PPARγmRNA表达的意义  

Significance of the expression of PPAR-γ mRNA in biliary tissue of rat biliary ischemia-reperfusion injury model of orthotopic liver transplantation

作　　者：裴红红[1] 张正良[1] 白郑海[1] 柏玲[1] 缪菲[1] 

机构地区：[1]西安交通大学医学院第二附属医院,西安710004

出　　处：《陕西医学杂志》2011年第2期141-144,共4页Shaanxi Medical Journal

基　　金：陕西省科技发展计划资助项目(2010K15-03-02)

摘　　要：目的:探讨PPARγ在大鼠自体原位肝移植胆道缺血再灌注损伤胆道组织中的表达情况以及意义。方法:40只SD大鼠随机分成假手术组(SO组)、缺血-再灌注组(I/R组)、罗格列酮组(ROS组)和GW9662组,每组10只。通过制作大鼠自体原位肝移植胆道缺血再灌注模型,应用PPARγ的配体罗格列酮(ROS)和拮抗剂GW9662作为干预,采用RT-PCR方法检测各组胆道组织中PPARγmRNA表达情况,应用图像分析系统进行灰度扫描,以各组β-actin条带的扫描值为标准,计算PPARγmRNA/β-actin吸光度比值,记录PPARγmRNA的相对表达量。结果:PPARγmRNA在SO组胆道组织中呈低表达;I/R组PPARγmRNA表达稍有增高,较SO组无显著差异;ROS组PPARγmRNA表达显著增加,与I/R组比较有显著差异(P<0.05);在GW9662组PPARγmRNA表达显著降低,与ROS组比较有显著差异(P<0.05)。结论:SD大鼠自体原位肝移植胆道缺血再灌注损伤各实验组胆道组织中存在PPARγmRNA表达,但各组表达量不同。配体罗格列酮可以活化原位肝移植胆道缺血再灌注损伤中肝外胆道组织中的PPARγ,但这种活化是PPARγ依赖性的,可被PPARγ的拮抗剂GW9662逆转。Objective：To explore the expression of PPARγ in biliary tissues of rats biliary ischemia-reperfusion injury model of orthotopic liver transplantation and its significance.Method： Forty Sprague-Dawley（SD） rats were randomly divided into four groups,with 10 in each： sham operation group（SO）,ischemia-reperfusion group（I/R）,Ros group and GW9662 group.We successfully made the rats biliary ischemia-reperfusion models of orthotopic liver transplantation,and interfered them with PPARγ ligand rosiglitazone,as well as its antagonist GW9962.By RT-PCR,We tested the expression of PPARγ mRNA in biliary tissues of each group.We scanned the gray scale by imaging analysis system,and took the scanning value of the β-actin as the standard clause in each group,and then calculated the absorbance ratio of PPARγ mRNA/β-actin,which was recorded as the relative expression of PPARγ mRNA.All results were analyzed by SPSS16.0.Results： The expression of PPARγ mRNA in SO was low,and though it was a little higher than that in I/R,there was no significance.Compared with I/R,the expression of PPARγ mRNA in Ros group was significantly increased（P0.05）,but in GW9662 group it was significantly declined when compared with that in Ros group.Conclusion： Though PPARγ mRNA expressed in all biliary tissues of SD rats biliary ischemia-reperfusion injury model of orthotopic liver transplantation in each group,there was difference among them.PPARγ,which expressed in biliary tissues of ischemia-reperfusion injury model of orthotopic liver transplantation,can be activated by its ligand rosiglitazone,and the activation,which is dependant on PPARγ,can be reversed by GW9662,the antagonist of PPARγ.

关 键 词：肝移植 胆道 再灌注 @过氧化物酶增殖体激活受体γ 

分 类 号：R657.4[医药卫生—外科学]