干细胞因子与白介素-3联合对小鼠骨髓细胞体外扩增作用及其造血重建的影响  

Influence of Stem Cell Factor Combined with Interleukin 3 on Expansion in Vitro of Murine Bone Marrow Cells and its Hematopoietic Reconstitution Capacity

作　　者：万岁桂[1] 刘聪艳[1] 路继莲[1] 孙雪静[1] 邵文硕[1] 田丁[1] 

机构地区：[1]首都医科大学宣武医院血液科

出　　处：《首都医科大学学报》1999年第3期170-173,共4页Journal of Capital Medical University

摘　　要：采用集落培养的方法研究了干细胞因子（ Ｓ Ｃ Ｆ） 联合白介素３（ Ｉ Ｌ３） 对小鼠５氟尿嘧啶（５ Ｆ Ｕ ） 处理３ ｄ 的骨髓细胞（ｄ３５ Ｆ Ｕ Ｂ Ｍ Ｃ） 的体外扩增作用及其对致死量照射小鼠造血重建功能的影响。 Ｓ Ｃ Ｆ 单独应用使体系中粒巨噬细胞集落形成单位（ Ｇ Ｍ Ｃ Ｆ Ｕ） 和高增殖潜能集落形成单位（ Ｈ Ｐ Ｐ Ｃ Ｆ Ｕ） 分别增加了（２４ ．２ ±６ ．１） 和（１ ．５ ±０ ．３） 倍（ Ｐ＜ ０ ．０５） 。 Ｓ Ｃ Ｆ 与 Ｉ Ｌ３ 联合应用时 Ｇ Ｍ Ｃ Ｆ Ｕ 和 Ｈ Ｐ Ｐ Ｃ Ｆ Ｕ 分别是新鲜ｄ３５ Ｆ Ｕ Ｂ Ｍ Ｃ 的（３０ ．５ ±９ ．５） 和（１３ ．６ ±４ ．５）倍（ 与单用 Ｓ Ｃ Ｆ 组比较 Ｐ 分别大于和小于０ ．０５） 。小鼠骨髓移植实验结果表明： Ｓ Ｃ Ｆ、 Ｓ Ｃ Ｆ＋ Ｉ Ｌ３ 体外扩增的ｄ３５ Ｆ Ｕ Ｂ Ｍ Ｃ 移植给经致死量照射的受体小鼠后，外周血细胞计数恢复时间比输新鲜ｄ３５ Ｆ Ｕ Ｂ Ｍ Ｃ 组缩短１ ～５ ｄ ， Ｓ Ｃ Ｆ＋ Ｉ Ｌ３组不仅血细胞计数恢复最快，并使移植所需的骨髓细胞数减少９０ ％ 以上。结果提示： Ｓ Ｃ Ｆ 单用对骨髓细胞有扩增作用，并且与 Ｉ Ｌ３ 有协同作用；体外扩增的骨髓?Investigation was carried out on the effects of stem cell factor (SCF) in combination with interleukin 3(IL 3) on expansion of mice 5FU treated 3 days′ bone marrow cells(d3 5FU BMC )in vitro using the method of colony culture and hematopoietic recovery in lethally irradiated mice transplanted with the expanded cells .The result demonstrated that the number of granulocyte macrophage colony forming units(GM CFU) and high proliferative potential colony forming units(HHP CFU) increased (24.2±6.1) fold and (1.5±0.3) fold( P <0.05) respectively compared with the control group when SCF was used alone.When SCF combined with IL 3,the number of GM CFU and HHP CFU was ( 30.5± 9.5 ) ( P >0.05 compared with SCF alone)and (13.6±4.5) times of that of the control group( P <0.05 compared with SCF alone). The result of mice bone marrow cells transplantation experiment demonstrated that the number of recipients’ peripheral blood cells which received lethally irradiation transplanted with expanded d3 5FU BMC with SCF,SCF+IL 3 recovered earlier by 1 5 days than the control group.Not only the number of the peripheral blood cells of SCF+IL 3 group recovered the earliest,but also the number of transplanted cells were reduced more than 10 folds. The result suggested that SCF had an effect on expansion of mice bone marrow cells in vitro.There was synergistic effect between SCF and IL 3.The expanded bone marrow cells could accelerate early hematopoietic reconstitution and reduce the number of transplanted cells.

关 键 词：造血生长因子 造血重建 干细胞因子 IL-3 

分 类 号：R457.7[医药卫生—治疗学]