用SCGE技术检验铬污染对鲫鱼肾细胞DNA损伤的研究  

Study on the Effects of Chromium (Cr+3)on DNA Damaging in Carp (Carassius auratus)Kidney Cells

作　　者：赵守城 张娟[1] 

机构地区：[1]华北煤炭医学院细胞生物学教研室

出　　处：《河北渔业》1999年第4期11-13,共3页

摘　　要：本文应用单细胞凝胶电泳(或彗星试验)研究了铬(Cr^(+3))对鲫鱼肾细胞DNA的损伤作用。结果表明,A组(0.8mg/l)、B组(1.6mg/l)和C组3.2mg/l)均可引起DNA的损伤。带有彗尾细胞的百分比值和彗尾长度均可依铬浓度增加而上升,呈现出剂量效应关系。本实验提示,铬浓度在不超过渔业水质标准的情况下,可引起鲫鱼肾细胞DNA的损伤。Using single cell gel - eletrophoresis (or comet assay)this paper presents the effects of chronium(Cr+3)on DNA breaks in.carp(Carassius auratus)kidney cells. The results show that a highly significant increase in DNA migration in DNA-damaged carp kidney cells was induced by three concentrations of chromium(Cr+3)compared with control (P< 0. 01). The percentage of DNA-damaged carp kidney cells was also increased. It is suggested that chromium can induce DNA damage and has toxic effect on carp kidney cells

关 键 词：氯化铬 鲫鱼 铬污染 DNA损伤 单细胞凝胶电泳 

分 类 号：S943.117[农业科学—水产养殖] X503.225[农业科学—水产科学]