CATSPER1蛋白与特发性弱精子症关系的研究  

CatSper1 protein and idiopathic asthenozoospermia

作　　者：武文斌[1] 李玉山[1] 冯晓霞[1] 王全先[1] 高学敏[1] 杨险峰[1] 潘周辉[1] 孙琳[1] 

机构地区：[1]郑州大学第三附属医院人类精子库,河南郑州450052

出　　处：《中华男科学杂志》2011年第2期110-114,共5页National Journal of Andrology

基　　金：河南省医学科技攻关计划项目(200903070)~~

摘　　要：目的:探讨CATSPER1蛋白在特发性弱精子症发病中的作用。方法:采用非连续密度梯度离心分离精子,免疫细胞化学技术检测CATSPER1蛋白在特发性弱精子症患者精子中的定位及表达变化;同时用蛋白印迹技术检测CATSPER1蛋白在正常对照组及轻度弱精子症组、中度弱精子症组和重度弱精子症组中表达的差异,进行统计学分析。结果:CATSPER1蛋白定位于精子鞭毛主段;与正常对照组相比,CATSPER1在弱精子症患者中的表达下降,差异具有显著性(t=2.188,P=0.042);CATSPER1蛋白在正常对照组、轻度弱精子症组、中度弱精子症组和重度弱精子症组中的相对含量分别为(0.806±0.266)、(0.669±0.207)、(0.505±0.214)、(0.295±0.162),与对照组相比,CATSPER1蛋白相对表达量在各弱精子症组均存在不同程度的下降,差异存在显著性(P<0.05)。前向运动精子(a+b级)百分率与CATSPER1蛋白的含量成正相关(r=0.633,P=0.000)。结论:CATSPER1蛋白表达下降或异常可能是导致特发性弱精子症发生的一个环节。Objective：To investigate the role of the cation channel of sperm 1 （CatSper1） protein in the pathogenesis of idiopathic asthenozoospermia. Methods： Sperm samples from patients with idiopathic asthenozoospermia were separated by Percoll discontinuous density gradients, and the distribution and expression of the CatSper1 protein were determined by immunocytochemistry. Western blotting was used to detect the different expressions of CatSper1 in the ejaculated sperm from the normal control, mild asthenozoospermia, moderate asthenozoospermia and severe asthenozoospermia groups, followed by statistical analyses. Results： The expression of CatSper1, located in the principle piece of the sperm tail, was reduced significantly in the samples from the idiopathic asthenozoospermia patients as compared with the normal controls （t=2.188, P=0.042）. The relative contents of the CatSper1 protein in the sperm of the control, mild asthenozoospermia, moderate asthenozoospermia and severe asthenozoospermia groups were 0.806±0.266, 0.669±0.207, 0.505±0.214 and 0.295±0.162, respectively, significantly decreased in the asthenozoospermia patients in comparison with the normal controls （P0.05）. There was a positive correlation between the percentage of progressively motile sperm and the relative content of the CatSper1 protein （r=0.633, P=0.000）. Conclusion： The decreased or abnormal expression of the CatSper1 protein may be a factor involved in the pathogenesis of idiopathic asthenozoospermia.

关 键 词：CATSPER1蛋白 特发性弱精子症 蛋白印迹 免疫细胞化学技术 

分 类 号：R698.2[医药卫生—泌尿科学]