生脉注射液对不同周龄大鼠睾丸扭转复位后睾丸损伤影响的研究  

Effects of Shengmai injection on testicular injury after torsion/detorsion in rats of different ages

作　　者：周益福[1] 倪春超[1] 郑婷婷[1] 徐乃晶[1] 王宇[1] 余晶晶[1] 张金萍[1] 高贵山[1] 

机构地区：[1]绍兴文理学院医学院临床医学系,浙江绍兴312000

出　　处：《中华男科学杂志》2011年第2期185-188,共4页National Journal of Andrology

基　　金：浙江省新苗人才计划项目(2008R40G218008);浙江省大学生科技创新项目(200810)~~

摘　　要：目的:探讨生脉注射液对不同周龄大鼠睾丸扭转复位后睾丸损伤影响的差异。方法:3、6、12周龄健康雄性SD大鼠各16只,随机分成睾丸扭转复位+注射生脉注射液组(实验组)和睾丸扭转复位+注射生理盐水组(对照组),每组8只,建立睾丸扭转动物模型(左侧阴囊切开,绕精索顺时针扭转睾丸720°2h),并于手术后24h处死,测定各组大鼠睾丸组织内总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性与丙二醛(MDA)含量。结果:与各自对照组比较,3、6周实验组大鼠左侧睾丸组织中T-AOC、SOD活性和MDA含量均无显著性改变(P>0.05);12周实验组大鼠左侧睾丸组织中SOD、T-AOC明显升高,而MDA含量显著降低,差异均具有显著性(P<0.05)。结论:生脉注射液对睾丸扭转复位后的缺血再灌注急性损伤有保护作用,但对不同周龄大鼠的再灌注损伤保护作用不同,存在年龄相关性差异,对较大周龄大鼠的急性保护作用较为明显。Objective：To investigate the different effects of Shengmai injection on testicular injury after testis torsion/detorsion in rats of different ages.Methods： Sixteen healthy male SD rats aged 3,6 and 12 weeks were equally randomized into an experimental group （testicular torsion/detorsion plus Shengmai injection） and a control group （testicular torsion/detorsion plus saline）.The rat models of testicular torsion were killed 24 h after surgery for the measurement of total antioxidant capacity （T-AOC）,superoxide dismutase （SOD） activity and malondialdehyde （MDA） content in the testis.Results： Compared with the controls,the 3-and 6-week-old rats of the experimental group showed no significant changes in T-AOC,SOD activity and MDA content （P 0.05）,while the 12-week-old experimental rats exhibited a remarkable increase in SOD and T-AOC and an obvious decrease in MDA content （P 0.05）.Conclusion： Shengmai injection has a protective effect against acute ischemia-reperfusion testicular injury after torsion/detorsion in rats,but the effect varies with the age,more obvious in older ones.

关 键 词：生脉注射液 睾丸扭转 鼠龄 总抗氧化能力 超氧化物歧化酶 丙二醛 大鼠 

分 类 号：R285.5[医药卫生—中药学]