小承气合剂促进结肠吻合术后吻合口组织愈合的实验研究  

Animal Experiment Study for The Efficacy of Xiaochengqi-Mixture on Promoting Healing of Colonic Stoma

作　　者：苏丹[1] 兰平[1] 任东林[1] 陈经宝[1] 林宏城[1] 

机构地区：[1]中山大学附属第六医院结直肠肛门外科,广东广州510655

出　　处：《中国普外基础与临床杂志》2011年第1期39-42,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家中医药管理局中医药留学回国人员科技活动择优资助课题项目(项目编号:2006LHR07)~~

摘　　要：目的评价小承气合剂对促进结肠吻合术后吻合口组织愈合的作用。方法选用Wistar大鼠40只,采用随机数字表法分为实验组(n=20)与对照组(n=20),2组均行结肠切除吻合术造模。实验组大鼠术后早期灌服小承气合剂,对照组大鼠同期灌服净化自来水。分别于术后第3、7和14天每组取相同数量大鼠进行剖腹探查,取吻合口及周围肉芽组织,检测羟脯氨酸含量和胶原纤维密度(Masson染色)。结果术后第3天,吻合口胶原已经开始增生,但纤维纤细,尚未形成束状纤维束,实验组大鼠羟脯氨酸含量及胶原纤维密度较对照组增高(P<0.05);术后第7天开始出现明显纤维化,但纤维束较细,纤维排列紊乱,实验组大鼠羟脯氨酸含量及胶原纤维密度明显高于对照组(P<0.01)。术后第14天,吻合口组织呈现广泛纤维化,且纤维束较粗,排列较整齐,2组大鼠羟脯氨酸含量及胶原纤维密度差异无统计学意义(P>0.05)。结论小承气合剂可促进术后胃肠吻合口愈合,可能有益于结肠吻合口漏的预防。Objective To evaluate the efficacy of Xiaochengqi-Mixture（XM） on promoting healing of colonic stoma.Methods Forty Wistar rats were divided into two groups randomly after colonectomy： experimental group（n=20） and control group（n=20）.In early postoperatively stage rats were given gastric administration of XM in the experimental group and pure water in the control group.On day 3,7,and 14 after establishment of animal models,laparotomy was performed in two groups of rats,respectively.Anastomotic stoma and surrounding tissues were harvested to detect the context of hydroxyproline and collagen fiber proportion by Masson dying.Results On day 3 after establishment of animal models,hyperplastic collagen with small fiber was observed while no fasciculus was found.Hydroxyproline context and collagen fiber proportion of rats were higher in experimental group than those in control group（P〈0.05）.On day 7 after operation,many fasciculuses were found in two groups of rats,hydroxyproline context and collagen fiber proportion of rats were higher in experimental group than those in control group（P〈0.01）.On day 14 after operation,fasciculuses became bigger and more regular in arrangement,but there was no significant difference between the two groups（P〈0.05）.Conclusion XM is capable of promoting healing of colonic stoma and might prevent the occurrence of anastomotic fistula.

关 键 词：小承气合剂 吻合口漏 组织愈合 羟脯氨酸 MASSON染色 胶原纤维 

分 类 号：R269[医药卫生—中西医结合]