SOX10在先天性巨结肠相关性小肠结肠炎发病中的作用  

The role of SOX10 in the pathogenesis of Hirschsprungs disease associated enterocolitis

作　　者：郑辉明[1] 向磊[2] 李宁[3] 韦佳[3] 郑帅玉[3] 邱银荣[3] 冯杰雄[3] 

机构地区：[1]泉州市第一医院小儿外科,362000 [2]华中科技大学同济医学院附属同济医院小儿外科研究室 [3]华中科技大学同济医学院附属同济医院小儿外科

出　　处：《中华小儿外科杂志》2011年第2期93-97,共5页Chinese Journal of Pediatric Surgery

基　　金：基金项目：2009年度福建省泉州市技术研究与开发项目（编号：2009229）;教育部留学回国人员启动基金（编号：2008-890）

摘　　要：目的探讨SOX10、潘氏细胞发育及分泌防御素-5与先天性巨结肠相关性小肠结肠炎的关系。方法收集50例先天性巨结肠病变肠管，根据术前是否发生小肠结肠炎分为HAEC组（n=14）和HD组（n=36），并以20例正常结肠标本作对照组。采用免疫组织化学方法观察结肠中防御素-5蛋白质表达、潘氏细胞发育情况以及SOX10蛋白质表达情况。采用实时荧光定量PCR技术检测防御素～5mRNA及Sox10mRNA表达情况。结果防御素-5在正常肠管中不表达，HAEC组和HD组在肠腺隐窝基底处呈不同程度阳性表达。但前者阳性区域平均光密度值明显增高（0．33±0．039比0．10±0．031，P〈0．05），HAEC组防御素-5mRNA亦呈显著增高趋势（2．72±0．80比0．78±0．21，P〈0．05）。对结肠组织同层切片进行潘氏细胞特异性产物溶菌酶免疫组化染色发现，对照组肠管中除1例存在弱阳性外其他均无阳性表达。HD组和HAEC组结肠中同样在隐窝基底处存在溶菌酶阳性细胞，可鉴别为化生的潘氏细胞，但HAEC组在发生率（78．6％）和细胞个数（2．97±0．80）明显高于HD组（27．8％，0．43±0．85）（P〈0．05）。SOX10免疫产物主要在结肠神经节细胞膜及胞浆中表达，对照组、HAEC组、HD组阳性区域的平均光密度值递减（0．75±0．041，0．61±0．048，0．35±0．025），差异具有统计学意义（P〈0．05），同时RT-PCR检测显示Soxl0mRNA在各组中的表达与蛋白质水平呈平行结果。结论SOXl0可能通过影响潘氏细胞发育及分泌防御素－5在先天性巨结肠相关性小肠结肠炎的发生发展中起一定作用。Objective To study the expression ot SOX10 and Human a-detensins-5 （HD-5）m Hirschsprung＇s disease associated enterocolitis（HAEC） and expore the possible relationship between SOX10 and HAEC. Methods Fifty pathological colons of Hirschsprung＇s disease （HD）were divided into HAEC group （n = 14） and HD group（n = 36） according to the presence of preoperative enterocolitis, Twenty normal colons as control group. The protein and mRNA expression of HD-5 and SOX 10 were measured by immunohistochemical staining and real-time quantitative PCR. Results Normal colons did not express HD-5 but positive expression of HD-5 was detected at the base of the crypts of Lieberkuhn in HAEC and HD groups in different degree. The mean optical density of HD-5 immuno- histochemical staining （0. 33 ± 0. 039 vs 0. 10 ± 0. 031）and HD-5 mRNA expression （2. 72 ± 0. 80 vs 0. 78 ± 0. 21） in HAEC group was apparently higher than those in HD group（P〈0. 05）. The expression of lysozyme, a specific product by Paneth Cell, on sequential sections was negative in control group except one sample. In HAEC group and HD group, positive expression of lysozyme can be seen in the crypts of Lieberkuhn, where the cells can be identified as Metaplastic Paneth cell, but the incidence （78. 6% vs 27. 8%） and the number of cells 2. 97 ± 0. 80 vs 0. 43 ± 0. 85） in HAEC group were obviously higher than those in HD group（2. 97 ± 0. 80 vs 0. 43 ± 0. 85） （P〈0. 05）. SOX10 was mainly located in the plasmalemma and cytoplasm of ganglion cell and its mean optical density in control group （0. 75 ± 0.041）, HAEC group（0.61 ± 0.048） and HD group（0. 35 ± 0. 025） were decrement, the difference between three groups were statistically significant （P〈 0. 05）. Meanwhile, Soxl0mRNA detected by real-time quantitative PCR indicated the parallel result. Conclusions SOX10 may be an important factor in the pathogenesis and development of HAEC.

关 键 词：SOX10 a防御素 潘氏细胞 先天性巨结肠相关性小肠结肠炎 

分 类 号：R726.5[医药卫生—儿科]