辣椒烟酰胺腺嘌呤二核苷酸磷酸基因(NADPH)在辣椒中的遗传转化及其抗病性鉴定  

Pepper Nicotinamide Adenine Dinucleotide Phosphate Gene (NADPH) Transformation into Pepper and Transgenic Plants Identification of Resistance to Phytophthora capsici

作　　者：包良帅[1] 巩振辉[1] 柴贵贤[1] 李大伟[1] 

机构地区：[1]西北农林科技大学园艺学院,杨凌712100

出　　处：《农业生物技术学报》2011年第1期45-50,共6页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.30571262;No.30771467);"十一五"国家科技支撑计划(No.2006BAD01A7);"13115"科技创新工程重大科技专项(No.2008ZDKG-03);教育部高校博士点基金(No.200807120007);国家高技术研究发展计划(863)(No.2009AA10Z104-6)资助

摘　　要：为了分析辣椒NADPH基因与辣椒疫病抗性之间的关系,明确其在辣椒抗疫病方面的作用,以辣椒NADPH基因为目的基因,利用载体pBI121构建了植物表达载体pBI121-NADPH,采用农杆菌介导法转化辣椒(Capsicum annuum L.)感病品种B12,获得了5个卡那霉素抗性转化株系。对卡那霉素抗性转化植株进行PCR和RT-PCR分子检测发现,这5个转化株系含有目的基因CanNADPH。应用辣椒疫病抗性离体叶片鉴定技术,鉴定转基因植株对疫霉菌(Phytophthora capsici)的抗病性,结果表明,转基因辣椒的发病率较非转基因植株降低了22.2%,其病情指数降低了46.5%,表明辣椒NADPH基因在辣椒抗疫病方面有重要作用。In order to analyze the relationship between the Pepper Nicotinamide Adenine Dinucleotide Phosphate Gene gene（canNADPH） and the resistance to Phytophthora capsici,make clear the role of canNADPH gene resistance to P. capsici,the pBI121-NADPH expression vector was constructed by the vector pBI121. Pepper （Capsicum annuum） susceptible cv. B12 was transformed with pBI121-NADPH by Agrobacterium tumefaciens-mediated transformation. Kanamycin-resistant regenerated lines were obtained,PCR and RT-PCR molecule detection confirmed that the five transgenic lines carried the target gene. The resistance of transgenic plants to P. capsici was identified by in vitro leaf technique. Results showed that the incidence of transgenic plants were reduced about 22.2% than those of the non-transgenic peppers,and the disease index of transgenic plants were also reduced 46.5%. The result shows that the canNADPH gene plays an important role at the resistance to P. capsici.

关 键 词：辣椒 烟酰胺腺嘌呤二核苷酸磷酸(NADPH)基因 遗传转化 疫霉菌 抗病性鉴定 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]