接种大豆孢囊线虫4号生理小种诱导GmHs1^(pro-1)基因的表达分析  

Inducing Expression Analysis of Gmhs1^(pro-1) by Inoculating Soybean Cyst Nematode 4 Race

作　　者：魏利[1,2] 李英慧[2] 卢为国[3] 常汝镇[2] 邱丽娟[2] 

机构地区：[1]南昌大学生命科学学院,南昌330031 [2]中国农业科学院作物科学研究所/农业部作物种质资源利用重点开放实验室,北京100081 [3]河南省农业科学院棉花油料作物研究所,郑州450002

出　　处：《农业生物技术学报》2011年第1期77-84,共8页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.30490251;30471096);国家高技术发展计划(863计划)项目(No.2006AA100104;2006AA10Z164;2006AA10A111);国家"十一五"科技支撑计划项目(No.2006BAD13B05);国家重点基础研究发展规划(973)项目(No.2004CB117203)资助

摘　　要：大豆孢囊线虫(Heterodera glycines,Soybean cyst nematode,SCN)是严重危害世界大豆生产的害虫,大豆对孢囊线虫的抗性由多个基因控制,发掘和利用抗病基因对于SCN抗病品种的有效选育至关重要。本研究以大豆(Glycine max)感病品种中黄13和抗病品系中品03-5373为研究材料,对包含线虫抗病基因相关结构域Hs1pro-1_N和Hs1pro-1_C的候选基因GmHs1pro-1的表达特征进行实时荧光定量PCR分析,以期明确该基因与SCN抗性之间的关系。研究发现接种SCN4号生理小种(SCN4)后1d该基因的相对表达量出现明显的上调,比对照样品高13.4倍,其后的各时间点其表达量仍然保持持续上调的趋势,在接种6d后其上调趋势有所减弱。方差分析表明,接种与未接种相对表达量之间的差异均达到显著(P<0.05)和极显著(P<0.01)水平,说明SCN4侵染条件下,GmHs1pro1基因被诱导表达,该基因对SCN4具有一定的抗性作用。Soybean cyst nematode （SCN） is a pest which can cause worldwide soybean yield losses seriously. The resistance of soybean to SCN is controlled by multiple genes. It is very important to detect the disease-resistant genes and breed the SCN disease-resistant cultivar. In order to understand the relationship between the gene and resistance to SCN,we analyzed the expression characteristics of candidate gene GmHslpro-1 on Soybean （Glycine max） resistant line Zhongpin03-5373 and susceptible cultivar Zhonghuang 13 using the Real-time PCR technique. We found that the relative expression of the gene raised after 1 d vaccinating SCN 4 Race （SCN 4） and it was 13.4 times higher than that of the control sample,it kept upward until inoculated 6 d. Analysis of variance showed that the relative expression of the gene was significant difference between inoculated and un-inoculated at significant difference （0.05 or 0.01 level）. It indicates that GmHslpro-1 may be induced to express in the conditions of SCN 4 infection,and the gene has a certain resistance to SCN 4.

关 键 词：大豆孢囊线虫 GmHs1pro-1基因 表达分析 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]