口蹄疫病毒整联蛋白受体αvβ6在绵羊体内的组织分布  

Exploring the Distribution of αvβ6 Integrin Receptor for Foot-and-mouth disease virus in Sheep Tissues

作　　者：薛霜[1] 独军政[1] 赵建勇[1] 高闪电[1] 常惠芸[1] 

机构地区：[1]中国农业科学院兰州兽医研究所国家口蹄疫参考实验室,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,兰州730046

出　　处：《农业生物技术学报》2011年第1期107-110,共4页Journal of Agricultural Biotechnology

基　　金：国家转基因生物新品种培育重大专项(No.2009ZX08007-008B、No.2009ZX08008-010B)资助

摘　　要：以间接免疫组化法(IHA)检测口蹄疫病毒(Foot-and-mouth disease virus,FMDV)整联蛋白αvβ6在健康绵羊(Ovis aries)不同组织器官中的表达分布。研究结果表明,在舌、鼻、唇、软腭、咽、喉头、气管、肺脏和蹄冠状带组织上皮细胞的细胞膜及细胞质内均出现了棕黄色的特异阳性反应物。结果表明,整联蛋白受体αvβ6受体广泛存在于健康绵羊体内的组织器官中,在呼吸系统以及蹄冠状带等多种FMDV易感组织器官的上皮细胞中表达量较高,且主要在细胞膜和细胞质中表达。To detect the expression and distribution of integrin αvβ6 of Foot-and-mouth disease virus（FMDV） in the tissues and organs of healthy sheeps（Ovis aries）,and explore the biological basis of tissue tropism in the FMDV infection,tissue profile of integrin αvβ6 in healthy sheeps was detected by indirect immunohistochemistry assay（IHA）. Results showed that buffy idio-positive reactant was appeared in cell membrane and cytoplasm of epithelium of sheep tongue,nose,lip,palatum molle,pharynx,larynx,trachea,lung and pedal coronary band,respectively. These results suggest that integrin αvβ6 receptor is generally expressed in tissues and organs of sheep,and mainly expressed in cell membrane and cytoplasm. Importantly,integrin αvβ6 is expressed at high levels in organs of respiratory system and pedal coronary band epithelium in sheeps.

关 键 词：口蹄疫病毒 整联蛋白αvβ6 间接免疫组织化学 组织分布 

分 类 号：S855.3[农业科学—临床兽医学]