N-甲基-D-天冬氨酸(NMDA)对大鼠下丘脑生长抑素分泌及其mRNA表达的影响  

N-methyl-D-aspartate(NMDA) Increases mRNA Expression and Secretion of the Hypothalamic Somatostatin

作　　者：熊吉敏[1] 周源[1] 张静波[1] 冯杰[1] 

机构地区：[1]浙江大学饲料科学研究所,教育部动物分子营养学重点实验室,杭州310029

出　　处：《农业生物技术学报》2011年第1期111-115,共5页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.30671524);浙江省重大科技攻关项目(No.2009C02007-1)资助

摘　　要：N-甲基-D-天冬氨酸(NMDA)是动物机体内存在的一种天然氨基酸衍生物,参与下丘脑-垂体生长轴的调控分泌作用。本实验选用SD大鼠(Rattus norvegicus)下丘脑,采用神经细胞体外培养模型来研究NMDA对下丘脑合成和分泌生长抑素(SS)的影响。结果表明,适量浓度的NMDA可显著促进下丘脑神经细胞SS的分泌及其mRNA的表达,明显提高细胞内的Ca2+水平,并使cAMP浓度降低,表明SS基因表达和分泌的增加可能主要是通过Ca2+和环腺苷酸(cAMP)共同介导实现的。N-methyl-D-aspartate （NMDA） is one of amino acid derivatives that exists in the body of animals and plays an important role in participating the effect of regulating secretion of hypothalamuic-putuitary-growth axis. The experiment was concluded to study the effect of NMDA on gene expression and secretion of somatostatin（SS） in the cultured rat（Rattus norvegicus） hypothalamus neuron cell and approached to mechanism of this process. Results showed that NMDA could significantly increase the gene expression and secretion of SS. Furthermore,NMDA could enhance the level of intracellular Ca2＋ and reduce the cAMP obviously,which suggested that NMDA regulates SS secretion and gene expression by Ca2＋ and cyclic adenosine monophosphate（cAMP） in hypothalamus neuron cells.

关 键 词：N-甲基-D-天冬氨酸(NMDA) 生长抑素(SS) 神经细胞 环腺苷酸(cAMP) CA2+ 

分 类 号：S852.4[农业科学—基础兽医学]