联合内皮祖细胞移植对小鼠骨髓移植预处理中肝脏内皮损伤的修复作用  

The repair function of united endothelial progenitor cells transplantation on injured liver endothelimn by bone marrow transplantation conditioning

作　　者：齐昆明[1] 许世娟[1] 贾路[1] 宋国梁[1] 闫志凌[1] 陈翀[1] 徐开林[1] 曾令宇[1] 

机构地区：[1]徐州医学院附属医院血液科徐州医学院移植免疫实验室,江苏省徐州市221002

出　　处：《中华器官移植杂志》2011年第2期82-86,共5页Chinese Journal of Organ Transplantation

基　　金：基金项目：国家自然科学基金（81070446）;江苏省科技创新人才项目（BK201）7504）

摘　　要：目的研究联合内皮祖细胞（EPC）移植对小鼠骨髓移植预处理中肝脏内皮损伤的修复作用。方法将C57BL／6小鼠分为4组，每组10只。（1）正常对照组：小鼠不做任何处理，仅作为正常对照；（2）单纯照射组：单次给予全身照射（TBI）预处理，不进行骨髓移植；（3）单纯移植组：给予单纯照射组相同的TBI预处理，TBI后4h内经小鼠尾静脉输注C57BL／6小鼠骨髓单个核细胞5×10^6／只；（4）联合移植组，小鼠的处理方式与单纯移植组相同，仅在骨髓移植的同时经尾静脉输注C57BL／6小鼠EPC5×10^5／只。TBI后第2、4、7、14、21天，检测各组小鼠肝脏重量的变化情况，并于TBI后第4、7、14、21天对各组小鼠肝脏进行组织病理学检查。结果单纯照射组、单纯移植组和联合移植组小鼠肝脏重量均于TBI后第2天开始明显增加，于第14天达到高峰，峰值分别为正常对照组的（1．65±0．15）倍（P〈0．05）、（1．61±0．06）倍（P〈0．05）和（1．11±0．40）倍（P〈0．05）；以后均呈下降趋势，第21天时单纯照射组和单纯移植组肝脏重量仍明显高于正常对照组（P〈0．05），但联合移植组小鼠肝脏重量已完全恢复正常。组织病理学检查显示，单纯照射组小鼠肝窦内皮损伤明显，肝细胞水肿及严重的炎症细胞浸润，第7天时肝细胞水肿、坏死较前明显加重，几乎无存活的肝血窦内皮细胞；第14天时单纯移植组小鼠肝窦内皮损伤较前有所减轻，但到第21天时仍未恢复正常；联合移植组小鼠第7天时肝窦内皮及肝细胞水肿、坏死程度均较轻，到第14天时已基本恢复正常。结论造血干细胞移植前的预处理会造成受者肝脏内皮损伤，且此损伤持续存在;移植时联合输注EPC能修复肝窦内皮的损伤。Objective To study the repair function of united endothelial progenitor cells （EPC） transplantation on injured liver endothelium by bone marrow transplantation （BMT） conditioning. Methods C57BL/6 mice were divided into four groups randomly., normal control group, without any treatment; irradiation alone group, administered a total body irradiation（TBI） pretreatment, without BMT~ （3） BMT alone group. C57BL/6 mice were infused with bone marrow mononuclearcells （MNC） 5 × 10^6/only through caudal vein not more than 4 h after the same TBI pretreatment as the irradiation alone group; united transplantation group： receiving the same way as the BMT alone group, but C57BL/6 mice were infused with EPC 5 × 10^5/only at the same time. Two, 4, 7, 14, and 21 days after the TBI, the changes of the liver weight were observed regularly. The histopathological examination of liver was done at the 4th, 7th, 14th, and 21st day after the TBI. Results In irradiation alone group, BMT alone group and united transplantation group the liver weight began to increase significantly on the day 2 and peaked at 14th day after the TBI, and the peaks were respectively （1.65 ±0. 15） times （P〈0. 05）, （1.61 ± 0. 06） times （P〈0. 05）, and （1.11 ± 0. 40） times （P〈0. 05） of those in normal control group. At the day 14, the liver weight in irradiation alone group, BMT alone group and united transplantation group began to decrease, and on the day 21 the liver weight in united transplantation group had been completely restored to normal level, however the liver weight in irradiation alone group and BMT alone group were still significantly heavier than that in normal control group （P〈0. 05）. Liver histopathologieal examination revealed that there were obvious sinusoidal endothelial cells （SEC） injury, hepatoeyte edema and severe inflammatory cell infiltration in irradiation alone group, and on the day 7 the hepatoeyte edema and necrosis were significantly worse than before, and almost no aliv

关 键 词：骨髓移植 内皮组细胞 移植预处理 损伤 小鼠 肝脏 

分 类 号：R457.7[医药卫生—治疗学]