弓形虫可溶性抗原混合液延长小鼠移植心脏存活时间  

Prolongation of mouse heart allograft survival by injecting T. gondii soluble tachyzoite antigen

作　　者：王升[1] 方正明[1] 黄霞[1] 蔡兰军[1] 余道武[1] 方泽民[1] 唐燕雷[1] 罗鲜樟[1] 宫念樵[1] 明长生[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所教育部/卫生部器官移植重点实验室,武汉430030

出　　处：《中华器官移植杂志》2011年第2期87-90,共4页Chinese Journal of Organ Transplantation

基　　金：基金项目：国家自然科学基金（30772052）

摘　　要：目的探讨弓形虫可溶性抗原混合液（STAgs）延长小鼠移植心脏存活时间的作用及其作用机制。方法通过在冰浴中超声粉碎弓形虫速殖子制备弓形虫STAgs。实验分为3组，每组受者9只。STAgs组和急性排斥反应（AR）组：供者为Balb／c小鼠，受者为C57BL／6小鼠，移植前4d两组受者分别皮下注射STAgs5μg和磷酸盐缓冲液100ul，同系对照组供、受者均为C57BL／6小鼠，术前未进行任何处理。分组后建立小鼠颈部异位心脏移植模型。术后观察移植心脏存活时间，术后第7天每组处死3只受者，获取移植心脏行病理学检查观察排斥反应，采用免疫组织化学检测移植心中CD4^＋和CD8^＋T淋巴细胞。结果同系移植组在观察终点100d时均存活，AR组和STAgs组移植心脏存活时间分别为（6．7±0．5）和（70．8±3．5）d，3组间两两比较，差异均有统计学意义（P〈0．05）。术后第7天，同系移植组、AR组和STAgs组移植心排斥反应分级分别为0级、Ⅲ～Ⅳ级和0～I级；免疫组织化学检测显示STAgs组CD4^＋和CD8^＋T淋巴细胞比例明显少于AR组，差异有统计学意义（P〈0．05）。结论弓形虫STAgs能显著延长小鼠移植心脏的存活时间，减轻移植心脏的排斥反应，具体机制可能与弓形虫STAgs可影响TH1／Tst2比例相关，也可能通过刺激机体产生脂氧素A4抑制树突状细胞活化发挥作用。Objective To investigate the effects of T. gondii soluble tachyzoite antigen （STAgs） on the survival time of mouse heart allograft and the possible mechanism. Methods The STAgs were prepared by pulverizing T. gondii tachyzoite with ultrasound on ice. Cervical heterotopic heart transplantations were done by using Balb/c mice as donors, and C57BL/6 mice as recipients. The recipients were classified randomly into three groups： syngeneic group, acute rejection group and STAgs-treated group. The recipients in acute rejection group and STAgs-treated group were injected subcutaneously with 0. 1 ml PBS and 0. 1 ml （5 μg） STAgs at the 4th day before transplantation respectively, and those in syngeneic group were not subjected to any treatment. The grafts were observed daily by cervical palpation, and the total cessation of cardiac contraction was defined as the endpoint. The heart allografts were harvested at the 7th day after transplantation for pathological examination and immunohistochemical staining for CD4^± T, CD8^± T. Results The recipients in syngeneic group were all alive at the 100th day after transplantation. The average survival time in acute rejection group and STAgs-treated group was （6.7 ± 0. 5） days and （70. 8 ± 3.5） days, respectively （P〈0. 05）. HE staining showed that the rejection on the 7th day after transplantation in syngeneic group, acute rejection group and STAgs-treated group was fallen into 0 degree, HI-IV degree and 0- I degree, respectively. Immunohistochemical staining revealed that the CD4^± T and CD8^±T were markedly down-regulated in STAgs-treated group as compared with those in acute rejection group. Conclusion T. gondii STAgs can significantly prolong the survival time of mouse heart allograft and inhibit the rejection probably by changing the ratio of TH 1/Tu 2, or inhibiting the effect of dendritic cells by inducing the lipoxin A4.

关 键 词：弓形虫属 可溶性抗原 心脏移植 小鼠 排斥反应 

分 类 号：R654.2[医药卫生—外科学]