检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:张艳梅[1] 陶晓倩[1] 时姗姗[1] 柳海燕[1] 卢坤刚[1] 韩雪峰[1] 姚兵[1]
机构地区:[1]南京军区南京总医院解放军检验医学研究所,南京理学硕士210002
出 处:《医学研究生学报》2011年第1期11-14,共4页Journal of Medical Postgraduates
基 金:国家自然科学基金(30770801);全军医药卫生科研基金(06H011)
摘 要:目的研究表明促性腺激素释放激素(gonadotrop in-releasing hormone,GnRH)可能对胰高血糖素具有调节作用,文中观察GnRH类似物对仓鼠胰高血糖素瘤细胞(In-R1-G9)胰高血糖素分泌的影响。方法通过W estern blot和免疫细胞化学的方法检测GnRH受体在In-R1-G9细胞中的表达和定位,然后分别用终浓度为0、0.1、10、1000 nmol/L的GnRH类似物处理培养的In-R1-G9细胞2 h,用放射免疫法分别检测对照组和实验组中胰高血糖素分泌量。结果 GnRH受体在In-R1-G9细胞中表达并定位在细胞膜上。低浓度的GnRH激动剂(GnRHa)(0.1 nmol/L)增强胰高血糖素分泌的作用不显著;而高浓度的GnRHa(10、1000 nmol/L)能显著增强胰高血糖素的分泌(P<0.05)。低浓度的GnRH拮抗剂(GnRHant)(0.1 nmol/L)降低胰高血糖素分泌的作用不显著;而高浓度的GnRHant(10、1000 nmol/L)能明显地抑制胰高血糖素的分泌(P<0.05)。结论促性腺激素释放激素类似物能以浓度依赖性的方式调节In-R1-G9细胞中胰高血糖素的分泌,这一过程可能通过GnRH受体作用。Objective Gonadotropin-releasing hormone(GnRH) could regulate the release of glucagon.The present study aimed to investigate the effect of GnRH analog on glucagon release in In-R1-G9 cells.Methods The expression and localization of the GnRH receptor(GnRHR) in In-R1-G9 cells were detected by Western blot and immunocytochemistry.Various concentrations of GnRH analog(GnRHa) or GnRH antagonist(GnRHant)(0,0.1,10 and 1000 nmol/L)were respectively added to the KRB buffer followed by incubation of the cells for 2 hours.At the end of the incubation period,the medium was collected,and glucagon secretion in the control and experimental groups was measured by radioimmunoassay.Results GnRHR was expressed and located on the surface of In-R1-G9 cells.Compared with the control group,higher concentrations(10 and 1000 nmol/L) of GnRHa markedly stimulated glucagon release(P0.05),while the lower concentration(0.1 nmol/L) exhibited no significant increasing effect on it(P0.05).Higher concentrations of GnRHant remarkably inhibited glucagon release(P0.05),while the lower concentration showed no significant inhibitory effect on it(P0.05).Conclusion GnRH analog regulates glucagon release in a concentration-dependent manner in In-R1-G9 cells,in which GnRHR seems to be involved.
关 键 词:促性腺激素释放激素类似物 In-R1-G9细胞 胰高血糖素
分 类 号:R335.6[医药卫生—人体生理学]
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.200