BMP受体在大鼠骨髓间充质细胞成骨中的作用  

Bone morphogenetic protein-2 (BMP-2) promotes the osteogenesis of rat bone marrow stromal cells

作　　者：何倩婷[1] 盛士虎[1] 凌励[1] 匡代军[1] 王安训[1] 

机构地区：[1]中山大学附属第一医院口腔科,广东广州510080

出　　处：《中国口腔颌面外科杂志》2011年第1期23-26,共4页China Journal of Oral and Maxillofacial Surgery

基　　金：广东省自然科学基金(9151008901000035);广东省科技计划项目(200813080701002)~~

摘　　要：目的:研究骨形成蛋白受体(BMP-R)在大鼠骨髓间充质细胞(BMSCs)成骨中的作用机制。方法:分离大鼠BMSCs,将原代细胞分为4组,分别应用普通培养基(CM)、成骨诱导培养基(OM)、骨形成蛋白-2(BMP-2)和OM+BMP-2诱导培养基培养。应用碱性磷酸酶(ALPase)活性和钙结节染色(Von Kossa法)检测成骨分化程度,RT-PCR检测骨形成蛋白受体(BMP-R)的表达情况。采用SPSS16.0软件包对数据进行单因素方差分析。结果:OM可诱导BMSCs成骨分化,表现出高碱性磷酸酶活性和基质矿化能力。BMP-2可提高OM诱导BMSCs成骨分化的能力;OM在早期即可诱导BMP-RⅠA和BMP-RⅡ的表达,而BMP-2诱导BMP-R较迟。结论:BMP-2可提高OM诱导BM-SCs分化成骨的能力,BMP-R在BMSCs分化成骨中起重要作用。PURPOSE：To Investigate the mechanism of bone morphogenetic protein receptor（BMP-R） on inducing the osteogenesis of rat bone marrow stromal cells.METHODS：BMSCs were harvested from rats and induced in controlmedium（CM）,osteogenic medium（OM）,BMP-2,and OM with BMP-2.Alkaline phosphatase（ALP） activity assay and VonKossa staining were used to assess the differentiation.BMP receptor（BMP-R） was also assessed using RT-PCR.SPSS 16.0software package was used for data analysis with one-way ANOVA.RESULTS：BMSCs underwent osteogenic differentiation in OM group,showing high level of ALP activity and matrix mine ralization.BMP-2 increased osteogenesis of BMSCs induced by OM.Significant BMP-RⅠA,BMP-RⅡ expression could be detected in BMSCs in OM after 3 daysof stimulation,while BMP receptors were later detected in BMSCs in BMP-2.CONCLUSIONS：BMP-2 can increaseosteogenesis of BMSCs induced by OM.BMP receptors have a strong effect on the osteogenic differentiation of BMSCs.

关 键 词：骨形成蛋白-2 BMP-2 骨形成蛋白受体 骨髓间充质细胞 骨形成 BMP信号 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]